Suprageneric classification of thermoactinomyces vulgaris by nucleotide sequencing of 5S ribosomal RNA

Abstract

The 5S rRNA nucleotide sequence of Thermoactinomyces vulgaris was determined and compared with published sequences of representative Gram-positive bacteria. The primary and secondary structure of the sequence is of the type characteristic of Gram-positive bacteria that have DNA with a low proportion of guanine plus cytosine. It was evident from the phylogenetic trees that T. vulgaris has little in common with actinomycetes but is related to the genus Bacillus, showing a moderately high relationship with B. stearothermophilus. The taxonomic implications of these relationships are discussed and an emended description of the family Bacillaceae is given.