DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D.M. | ko |
dc.contributor.author | Kim, G.J. | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-02-27T07:29:02Z | - |
dc.date.available | 2013-02-27T07:29:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-09 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.16, no.1, pp.11 - 16 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67257 | - |
dc.description.abstract | A bacterium Pseudomonas sp. KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin. The half-life of immobilized whole cell D-hydantoinase was found to be about 50 hrs. In order to increase the operational stability of immobilized whole cell D-hydantoinase, a carbon or nitrogen source was supplied with the reaction mixture in the continuous stirred tank reactor. As a sole source of carbon, glycerol was found to be most effective, and the activity of immobilized whole cell enzyme was maintained stably during 7 days when O.1%(W/V) glycerol solution was provided. In the case of nitrogen source, supplying of O.1%(W/V) yeast extract prolonged the half-life of immobilized whole cell D-hydantoinase to about 25 days. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | AMINO-ACIDS | - |
dc.title | Enhancement of operational stability of immobilized whole cell D-hydantoinase | - |
dc.type | Article | - |
dc.identifier.wosid | A1994MU96100003 | - |
dc.identifier.scopusid | 2-s2.0-0028014309 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 11 | - |
dc.citation.endingpage | 16 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kim, D.M. | - |
dc.contributor.nonIdAuthor | Kim, G.J. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
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