Studies are made of the turbulent separation bubble in a two-dimensional semi-infinite blunt plate aligned to a uniform free stream when the oncoming free stream contains a pulsating component. The discrete-vortex method is applied to simulate this flow situations because this approach is effective to represent the unsteady motions of turbulent shear layer and the effect of viscosity near the solid surface. The two key external paramenters in the free stream, i.e., the amplitude of pulsation, A, and the frequency parameter St[=fH/ $U_{1}$], are dealt with in the present numerical computations, A particular frequency gives a minimum reattachment which is related to the drag reduction and the most effective frequency is dependent on the most amplified shedding frequency. The turbulent flow structure is scrutinized. A comparison between the unperturbed flow and the perturbed at the particular frequency of the minimum reattachment length of the separation bubble suggests that the large-scale structure is associated with the shedding frequency and the flow instabilities.