Synthesis of Branched Oligosaccharides from Starch by Two Amylases Cloned from Bacillus licheniformis

Abstract

An E. coli cell extract containing thermostable a-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Ale) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.