SERUM CAN ACT AS A SHEAR PROTECTING AGENT IN AGITATED HYBRIDOMA CELL-CULTURES

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dc.contributor.authorLee, Gyun-Minko
dc.contributor.authorSavinell, JMko
dc.contributor.authorPalsson, BOko
dc.date.accessioned2013-02-25T18:44:23Z-
dc.date.available2013-02-25T18:44:23Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1989-12-
dc.identifier.citationHYBRIDOMA, v.8, no.6, pp.639 - 645-
dc.identifier.issn0272-457X-
dc.identifier.urihttp://hdl.handle.net/10203/64427-
dc.description.abstractHybridoma cells, S3H5/γ2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.-
dc.languageEnglish-
dc.publisherMary Ann Liebert Inc.-
dc.titleSERUM CAN ACT AS A SHEAR PROTECTING AGENT IN AGITATED HYBRIDOMA CELL-CULTURES-
dc.typeArticle-
dc.identifier.wosidA1989CD23700007-
dc.identifier.scopusid2-s2.0-0024853086-
dc.type.rimsART-
dc.citation.volume8-
dc.citation.issue6-
dc.citation.beginningpage639-
dc.citation.endingpage645-
dc.citation.publicationnameHYBRIDOMA-
dc.identifier.doi10.1089/hyb.1989.8.639-
dc.contributor.localauthorLee, Gyun-Min-
dc.contributor.nonIdAuthorSavinell, JM-
dc.contributor.nonIdAuthorPalsson, BO-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
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