A diagnostic method of anisotropy and asymmetry in rotor systems utilizing the two-sided directional spectra of the operating responses has been presented and tested with a laboratory flexible rotor-bearing system. The experimental results show that the directional spectra can be effectively used for the diagnosis of anisotropy and/or asymmetry in rotor systmes by the investigation of -1X and +2X components in the directional spectrum of unbalance and gravity responses.