Stability of the Lipase Immobilized on DEAE-Sephadex for Continuous Lipid Hydrolysis in Organic Solvent
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | D. Yang | ko |
| dc.contributor.author | Rhee, Joon Shick | ko |
| dc.date.accessioned | 2013-02-25T16:16:23Z | - |
| dc.date.available | 2013-02-25T16:16:23Z | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.issued | 1991 | - |
| dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.13, no.8, pp.553 - 558 | - |
| dc.identifier.issn | 0141-5492 | - |
| dc.identifier.uri | http://hdl.handle.net/10203/63475 | - |
| dc.description.abstract | Continuous hydrolysis reaction was carried out in glass-column by using lipase from Candida rugosa immobilized on DEAE-Sephadex A50. Substrate olive oil dissolved in hydrophobic organic solvent (isooctane) was supplied into the reactor with a concurrent stream of aqueous buffer. Stability of the immobilized enzyme was greatly increased in higher substrate concentration. Glycerol added in the aqueous stream showed stabilizing effect against the organic solvent; half life was extended from 220 hrs to 450 hrs by 15% glycerol supplemented at 30-degrees-C. | - |
| dc.language | English | - |
| dc.publisher | Springer | - |
| dc.subject | OLIVE OIL | - |
| dc.subject | SYSTEMS | - |
| dc.subject | FAT | - |
| dc.title | Stability of the Lipase Immobilized on DEAE-Sephadex for Continuous Lipid Hydrolysis in Organic Solvent | - |
| dc.type | Article | - |
| dc.identifier.wosid | A1991GD28100004 | - |
| dc.identifier.scopusid | 2-s2.0-0025779546 | - |
| dc.type.rims | ART | - |
| dc.citation.volume | 13 | - |
| dc.citation.issue | 8 | - |
| dc.citation.beginningpage | 553 | - |
| dc.citation.endingpage | 558 | - |
| dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
| dc.identifier.doi | 10.1007/BF01033408 | - |
| dc.contributor.nonIdAuthor | D. Yang | - |
| dc.type.journalArticle | Article | - |
| dc.subject.keywordPlus | OLIVE OIL | - |
| dc.subject.keywordPlus | SYSTEMS | - |
| dc.subject.keywordPlus | FAT | - |
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