개 gastrin 유전자의 클로닝과 exon-intron의 제도Cloning and Exon-intron Mapping of Canine Gastrin Gene

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The canine gastin gene was isolated from a genomic library. Screening of about 500,000 recombinant phages was carried out by in situ plaque hybridization employing a canine gastrin cDNA clone as a probe. As a result two different genomic clones were obtained. These contain 13-kb and 15-kb genomic DNA fragments that share at least 11-kb DNA. Comparison of the determined genomic sequences with the cDNA sequence revealed the structure of three exons and two introns, which comprise 2.3 kb in total. It resembles the human gastrin gene structure. However, the canine intron I is only 1.7 kb long, about half the size of the human intron I.
Publisher
생화학분자생물학회
Issue Date
1990
Language
English
Citation

KOREAN BIOCHEMICAL JOURNAL, v.23, no.1, pp.69 - 74

ISSN
1125-8687
URI
http://hdl.handle.net/10203/58393
Appears in Collection
BS-Journal Papers(저널논문)MSE-Journal Papers(저널논문)
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