DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lees-Miller, James P. | ko |
dc.contributor.author | Goodwin, Leslie O. | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-02-25T00:11:02Z | - |
dc.date.available | 2013-02-25T00:11:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1990-04 | - |
dc.identifier.citation | MOLECULAR AND CELLULAR BIOLOGY, v.10, no.4, pp.1729 - 1742 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | http://hdl.handle.net/10203/58304 | - |
dc.description.abstract | cDNA clones encoding three novel tropomyosins, termed TMBr-1, TMBr-2, and TMBr-3, were isolated and characterized from a rat brain cDNA library. All are derived from a single gene, which was previously found to express striated muscle α-tropomyosin and a r of other tropomyosin isoforms via an alternative splicing mechanism (N. Ruiz-Opazo and B. Nadal-Ginard, J. Biol. Chem. 262: 4755-4765, 1987; D.F. Wieczorek, C.W.J. Smith, and B. Nadal-Ginard, Mol. Cell. Biol. 8: 679-694, 1988). The derived amino acid sequences revealed that TMBr-1 contains 281 amino acids, TMBr-2 contains 251 amino acids, and TMBr-3 contains 245-amino acids. All three proteins contain a region that is identical to amino acids 81 through 258 of skeletal muscle α-tropomyosin. TMBr-1 is identical to striated muscle α-tropomyosin from amino acids 1 through 258 but contains a novel COOH-terminal region from amino acids 259 through 281. TMBr-2 and TMBr-3 both contain identical NH2-terminal sequences from amino acids 1 through 44 which were found to be expressed from a novel promoter. TMBr-3 contains the same COOH-terminal region as TMBr-1, whereas TMBr-2 contains a second novel COOH-terminal region. The genomic organization of the exons encoding TMBr-1, TMBr-2, and TMBr-3 were determined. These studies revealed a previously uncharacterized promoter located in the internal region of the α-TM gene as well as two novel COOH-terminal coding exons. The α-TM gene is a complex transcription until containing 15 exons including two alternative promoters, two internal mutually exclusive exon cassettes, and four alternatively spliced 3 exons that encode four different COOH-terminal coding regions. A total of nine distinct mRNAs are known to be expressed from the α-TM gene in a cell type-specific manner in tissues such as striated muscle, smooth muscle, kidney, liver, brain, and fibroblasts. The mRNAs encoding TMBr-1, TMBr-2, and TMBr-3 were found to be expressed only in brain tissue, with TMBr-3 being expressed at much greater levels than TMBr-1 and TMBr-2. The individual structural characteristics of each brain α-tropomyosin isoform and their possible functions are discussed. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Three novel brain tropomyosin isoforms are expressed from the rat α-tropomyosin gene through the use of alternative promoters and alternative RNA processing | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-0025247677 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 1729 | - |
dc.citation.endingpage | 1742 | - |
dc.citation.publicationname | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Lees-Miller, James P. | - |
dc.contributor.nonIdAuthor | Goodwin, Leslie O. | - |
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