CHARACTERISTICS OF A NOVEL HIGH-VISCOSITY POLYSACCHARIDE, METHYLAN, PRODUCED BY METHYLOBACTERIUM-ORGANOPHILUM

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A new anionic high molecular weight polysaccharide, Methylan, was produced by Methylobacterium organophilum from methanol as a sole source of carbon and energy under the specific culture conditions. By GPC and light scattering, the molecular weight was determined to be 2-4 x 10(6) dalton and the distribution of molecular weight was very homogeneous. Methylan was composed of carbohydrate (80%), uronic acid (12%), protein (6%) and pyruvic acid (5%). The sugar composition of Methylan was identified as glucose, galactose and mannose with the approximate molar ratio of 2:3:2. Methylan solution showed a pseudoplastic non-Newtonian fluid property at the concentration above 0.05%. At the concentration of 1% Methylan solution, the consistency index was 18,000 centipoise which was almost 10 times higher than that of Xanthan and the flow behavior index was 0.15.
Publisher
SPRINGER
Issue Date
1991-06
Language
English
Article Type
Article
Keywords

EXTRACELLULAR POLYSACCHARIDE; METHANOL

Citation

BIOTECHNOLOGY LETTERS, v.13, no.6, pp.417 - 420

ISSN
0141-5492
DOI
10.1007/BF01030993
URI
http://hdl.handle.net/10203/56825
Appears in Collection
BS-Journal Papers(저널논문)
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