Morphologic evaluation and integrin expression profile of renal tubular cells cultured from percutaneous renal biopsy specimen

Cited 3 time in webofscience Cited 0 time in scopus
  • Hit : 655
  • Download : 2
Kidney biopsy is an indispensible procedure for making a pathologic diagnosis of renal diseases by fixing and staining the biopsy specimen. However, it is not a routine procedure to culture the cells from a renal biopsy specimen directly, or to utilize the cultured cells for any kind of diagnostic or functional evaluation. In this study, primary culture of the renal tubular epithelial cells was tried from a piece of percutaneous kidney biopsy specimen. Successive passages of the cells were possible until fourth passage. With these cells, morphologic characteristics of the cultured cells and integrin expression profiles were investigated. On light and electron microscopy, these cells were characterized by the cobblestone-like growth, presence of microvilli and tight junction, and the preservation of polarity. Immunohistochemical studies demonstrated the epithelial nature of these cells and particularly their differentiation from renal tubular epithelial cells, of either proximal or distal nephronic segment. The integrin profile confirms the epithelial nature of the cell. We hope that our results facilitate the understanding of pathophysiology of renal tubular cells from the patient directly.
Publisher
Informa Healthcare
Issue Date
2001
Language
English
Article Type
Article
Keywords

EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; KIDNEY; SUBUNITS; DISEASE; GROWTH

Citation

RENAL FAILURE, v.23, no.1, pp.21 - 29

ISSN
0886-022X
DOI
10.1081/JDI-100001280
URI
http://hdl.handle.net/10203/5448
Appears in Collection
ITM-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 3 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0