DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | LEE, ES | ko |
dc.contributor.author | MOGOE, T | ko |
dc.contributor.author | LEE, KK | ko |
dc.contributor.author | FUKUI, Y | ko |
dc.date.accessioned | 2008-06-03T06:10:07Z | - |
dc.date.available | 2008-06-03T06:10:07Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-09 | - |
dc.identifier.citation | THERIOGENOLOGY, v.44, no.4, pp.507 - 516 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | http://hdl.handle.net/10203/4857 | - |
dc.description.abstract | This study was conducted to investigate whether human leukemia inhibitory factor (hLIF) improves the subsequent development of IVF-derived bovine morulae and blastocysts. To obtain IVF-derived bovine morulae, ova were matured and fertilized in vitro and cultured in 0.5 ml of synthetic oviduct fluid (SOF) medium supplemented with 10% human serum (HS) for 5 d at 39 degrees C under a gas atmosphere of 5% CO2, 5% O-2, 90% N-2. Morulae and early blastocysts at Day 5 of culture were cultured in 0.5 me of SOF medium with or without 5000 U/ml recombinant hLIF for 2 or 3 d (2 groups). To investigate the effect of addition of hLIF on the subsequent development of morulae, SOF medium was supplemented with 8 mg/ml BSA instead of HS. To test whether hLIF affects the subsequent development of IVF-derived bovine blastocysts, only good blastocysts that developed from SOF medium with or without hLIF at Days 7 and 8 of culture were frozen by a conventional slow freezing method and again cultured in SOF medium with or without the addition of hLIF for 3 d after thawing (4 groups). Survival of frozen-thawed bovine embryos was evaluated for re-expansion and hatching of blastocysts during 3 d of culture. There was no significant difference in the developmental rate of Day 5 embryos to blastocysts between those cultured with (47.8%) and without (47.6%) addition of hLIF, However, the addition of hLIF before freezing significantly increased the hatching rate of IVF-derived bovine morulae (P<0.05), whereas addition of hLIF after thawing did not increase the subsequent development of blastocysts, These results suggest that hLIF added at the Day 5 morula stage may contribute to bovine embryonic development through the hatching process. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | BUTTERWORTH-HEINEMANN | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | FACTOR LIF | - |
dc.subject | INVITRO | - |
dc.subject | EXPRESSION | - |
dc.subject | OOCYTES | - |
dc.subject | FERTILIZATION | - |
dc.subject | IMPLANTATION | - |
dc.subject | EMBRYOS | - |
dc.subject | CULTURE | - |
dc.subject | CELLS | - |
dc.title | Effect of human leukemia inhibitory factor on in vitro development of IVF-derived bovine morulae and blastocysts | - |
dc.type | Article | - |
dc.identifier.wosid | A1995RQ60300005 | - |
dc.identifier.scopusid | 2-s2.0-0000786582 | - |
dc.type.rims | ART | - |
dc.citation.volume | 44 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 507 | - |
dc.citation.endingpage | 516 | - |
dc.citation.publicationname | THERIOGENOLOGY | - |
dc.identifier.doi | 10.1016/0093-691X(95)00222-T | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | LEE, ES | - |
dc.contributor.nonIdAuthor | MOGOE, T | - |
dc.contributor.nonIdAuthor | LEE, KK | - |
dc.contributor.nonIdAuthor | FUKUI, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | HLIF | - |
dc.subject.keywordAuthor | FREEZING | - |
dc.subject.keywordAuthor | IN VITRO DEVELOPMENT | - |
dc.subject.keywordAuthor | CATTLE | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | FACTOR LIF | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OOCYTES | - |
dc.subject.keywordPlus | FERTILIZATION | - |
dc.subject.keywordPlus | IMPLANTATION | - |
dc.subject.keywordPlus | EMBRYOS | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | CELLS | - |
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