DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | YAMASHINA, H | ko |
dc.contributor.author | KOYAMA, N | ko |
dc.contributor.author | LEE, KK | ko |
dc.contributor.author | FUKUI, Y | ko |
dc.date.accessioned | 2008-06-03T06:07:58Z | - |
dc.date.available | 2008-06-03T06:07:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-09 | - |
dc.identifier.citation | THERIOGENOLOGY, v.42, no.4, pp.645 - 654 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | http://hdl.handle.net/10203/4856 | - |
dc.description.abstract | Factors affecting viability of IVF-derived bovine blastocysts after freezing and thawing were investigated. A total of 1,101 ova matured and fertilized in vitro were cultured under 2 different conditions, 1) in TCM-199 on granulosa cell monolayers at 5% CO2 in air and 2) in synthetic oviduct fluid (SOF) medium without somatic cell support at 5% CO2, 5% O-2, 90% N-2. All blastocysts that developed from the 2 different culture systems were individually classified into 4 grades of embryo quality and were then frozen by conventional slow freezing. Developmental rates of the IVF-derived ova to blastocysts and the survival rates of the frozen-thawed blastocysts were not different between the SOF medium (16 and 49%) and the co-culture system (13 and 61%, respectively). Survival of frozen-thawed blastocysts was affected by embryo quality in both the SOF and co-culture systems (P<0.001). Blastocysts produced in vitro were also individually classified into 3 developmental stages and were then cultured for 3 d in the co-culture system with granulosa cells after freezing and thawing. There was a difference in the survival rate of frozen-thawed embryos between blastocyst developmental stages (early vs mid, P<0.05; mid vs expanded, P<0.01; early vs expanded, P<0.001). The post-thawing survival rate of blastocysts frozen at Day 7 (62%) of culture was higher compared with that of Day 8 (45%), but there was no difference in survival rate between Day 7 and 8 of culture. The results indicate that the quality and developmental stage of blastocysts are important factors influencing their survival after freezing and thawing. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | BUTTERWORTH-HEINEMANN | - |
dc.subject | FERTILIZED INVITRO | - |
dc.subject | FOLLICULAR OOCYTES | - |
dc.subject | CATTLE EMBRYOS | - |
dc.subject | CUMULUS CELLS | - |
dc.subject | CO-CULTURE | - |
dc.subject | IN-VITRO | - |
dc.subject | FROZEN | - |
dc.subject | VITRIFICATION | - |
dc.subject | MATURATION | - |
dc.subject | CALVES | - |
dc.title | Effects of quality and developmental stage on the survival of IVF-derived bovine blastocysts cultured in vitro after freezing and thawing | - |
dc.type | Article | - |
dc.identifier.wosid | A1994PF72800009 | - |
dc.type.rims | ART | - |
dc.citation.volume | 42 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 645 | - |
dc.citation.endingpage | 654 | - |
dc.citation.publicationname | THERIOGENOLOGY | - |
dc.identifier.doi | 10.1016/0093-691X(94)90381-R | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | YAMASHINA, H | - |
dc.contributor.nonIdAuthor | KOYAMA, N | - |
dc.contributor.nonIdAuthor | LEE, KK | - |
dc.contributor.nonIdAuthor | FUKUI, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CULTURE SYSTEMS | - |
dc.subject.keywordAuthor | EMBRYO QUALITY | - |
dc.subject.keywordAuthor | DEVELOPMENTAL STAGE | - |
dc.subject.keywordAuthor | FREEZING | - |
dc.subject.keywordAuthor | CATTLE | - |
dc.subject.keywordPlus | FERTILIZED INVITRO | - |
dc.subject.keywordPlus | FOLLICULAR OOCYTES | - |
dc.subject.keywordPlus | CATTLE EMBRYOS | - |
dc.subject.keywordPlus | CUMULUS CELLS | - |
dc.subject.keywordPlus | CO-CULTURE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | FROZEN | - |
dc.subject.keywordPlus | VITRIFICATION | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | CALVES | - |
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