DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Choi, Yang-Kyu | - |
dc.contributor.advisor | 최양규 | - |
dc.contributor.author | Jang, Dong-Yoon | - |
dc.contributor.author | 장동윤 | - |
dc.date.accessioned | 2011-12-14T02:02:37Z | - |
dc.date.available | 2011-12-14T02:02:37Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255541&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/38372 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 전기및전자공학전공, 2006.2, [ ii, 49 p. ] | - |
dc.description.abstract | In this thesis, label-free electrical detection of protein-ligand binding using a vertical gold nanogap is presented. A sub-lithographic vertical gold nanogap was created using a sacrificial ultra-thin film deposited by atomic layer deposition (ALD) in a process similar to the formation of a cantilever in MEMS processing. Due to the atomic precision of the sacrificial $Al_2O_3$ thickness by ALD, a 7-nm nanogap was successfully fabricated. After binding streptavidin to biotin on the gold surface, a DC electrical current was measured for various DC voltages. A dramatic DC current increase was observed in the case of biotin-streptavidin binding in comparison with the other two cases: a control group filled with air in nanogap and a biotin-only binding group. There was minimal current change in the cases of the biotin-PBST group, the biotin-BSA group, and the biotin-saturated streptavidin group, as compared with the biotin-streptavidin group. At a 0.1μg/ml concentration of streptavidin (1.5 nM), the current difference before and after the protein binding was amplified by approximately 3000-fold. Also, the detection sensitivity of the vertical nanogap as the gap size varied was investigated. This arrayable, two-terminal microdevice can be used for a wide range of detection applications in the analysis of other biomolecules and for nucleic acid hybridization. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | ALD | - |
dc.subject | Nanogap | - |
dc.subject | Label-free detection | - |
dc.subject | label이 필요없는 감지방법 | - |
dc.subject | 원자적 수준의 박막증착 | - |
dc.subject | 나노갶 | - |
dc.subject | ALD | - |
dc.subject | Nanogap | - |
dc.subject | Label-free detection | - |
dc.subject | label이 필요없는 감지방법 | - |
dc.subject | 원자적 수준의 박막증착 | - |
dc.subject | 나노갶 | - |
dc.title | (A) study of label-free electrical detection of protein-ligand binding with vertical gold nanogap | - |
dc.title.alternative | Vertical gold nanogap을 이용하여 protein-ligand binding을 electrical detection 하는 방법에 대한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 255541/325007 | - |
dc.description.department | 한국과학기술원 : 전기및전자공학전공, | - |
dc.identifier.uid | 020043506 | - |
dc.contributor.localauthor | Choi, Yang-Kyu | - |
dc.contributor.localauthor | 최양규 | - |
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