DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moon, JaeHoon | ko |
dc.contributor.author | Park, Jong Kwan | ko |
dc.contributor.author | Park, Bu Young | ko |
dc.contributor.author | Jeon, Hyung Jin | ko |
dc.contributor.author | Choi, Gi Sub | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2024-09-12T03:00:06Z | - |
dc.date.available | 2024-09-12T03:00:06Z | - |
dc.date.created | 2024-09-12 | - |
dc.date.issued | 2024-06 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.29, no.3, pp.505 - 512 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/322933 | - |
dc.description.abstract | Pertactin (PRN), a non-fimbrial outer membrane protein of Bordetella pertussis, is the limiting component of the acellular pertussis vaccine because of its low concentration. This study aimed to develop a large-scale urea-based process for PRN extraction from B. pertussis. Cell pellet processing conditions, including freezing and thawing, were found to substantially affect PRN yield. A single cycle of rapid freezing of the cell pellet at - 30 degrees C with slow thawing at 5 +/- 3 degrees C resulted in up to fivefold higher PRN yield than condition without freezing and thawing. The search for urea treatment conditions was also conducted, and 5 M urea treatment for 2 h was the optimal condition. The developed urea-based process was applied to 50 L culture scale, and residual impurities were removed by sequential anion exchange, hydrophobic interaction and gel filtration chromatography and resulted in PRN with a purity of over 95% at a yield of 33.2%. From 50 L culture broth, the final yield of PRN per cell pellet was 0.23 mg/g (wet weight). Thus, a large-scale production process for high-quality PRN from B. pertussis was developed based on urea extraction process. The results may serve as a reference for production of other membrane proteins. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Extraction of the outer membrane protein pertactin from Bordetella pertussis with urea for the production of acellular pertussis vaccine | - |
dc.type | Article | - |
dc.identifier.wosid | 001243610300001 | - |
dc.identifier.scopusid | 2-s2.0-85195492278 | - |
dc.type.rims | ART | - |
dc.citation.volume | 29 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 505 | - |
dc.citation.endingpage | 512 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/s12257-024-00028-2 | - |
dc.identifier.kciid | ART003100876 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Park, Jong Kwan | - |
dc.contributor.nonIdAuthor | Park, Bu Young | - |
dc.contributor.nonIdAuthor | Jeon, Hyung Jin | - |
dc.contributor.nonIdAuthor | Choi, Gi Sub | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Pertactin | - |
dc.subject.keywordAuthor | Acellular pertussis vaccine | - |
dc.subject.keywordAuthor | Outer membrane protein | - |
dc.subject.keywordAuthor | Urea extraction | - |
dc.subject.keywordAuthor | Membrane protein purification | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | DIPHTHERIA | - |
dc.subject.keywordPlus | TETANUS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EFFICACY | - |
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