High-level succinic acid production by overexpressing a magnesium transporter in Mannheimia succiniciproducens

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dc.contributor.authorKim, Ji Yeonko
dc.contributor.authorLee, Jonganko
dc.contributor.authorAhn, Jung Hoko
dc.contributor.authorLee, Sang Yupko
dc.date.accessioned2024-09-09T08:00:07Z-
dc.date.available2024-09-09T08:00:07Z-
dc.date.created2024-09-09-
dc.date.created2024-09-09-
dc.date.issued2024-09-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, v.121, no.37-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10203/322836-
dc.description.abstractSuccinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Although the importance of magnesium (Mg2+) ion on SA production has been evident from our previous studies, the role of Mg2+ ion remains largely unexplored. In this study, we investigated the impact of Mg2+ ion on SA production and developed a hyper-SA producing strain of M. succiniciproducens by reconstructing the Mg2+ ion transport system. To achieve this, optimal alkaline neutralizer comprising Mg2+ ion was developed and the physiological effect of Mg2+ ion was analyzed. Subsequently, the Mg2+ ion transport system was reconstructed by introducing an efficient Mg2+ ion transporter from Salmonella enterica. A high-inoculum fed-batch fermentation of the final engineered strain produced 152.23 ± 0.99 g/L of SA, with a maximum productivity of 39.64 ± 0.69 g/L/h. These findings highlight the importance of Mg2+ ions and transportation system optimization in succinic acid production by M. succiniciproducens.-
dc.languageEnglish-
dc.publisherNational Academy of Sciences-
dc.titleHigh-level succinic acid production by overexpressing a magnesium transporter in Mannheimia succiniciproducens-
dc.typeArticle-
dc.identifier.scopusid2-s2.0-85203420205-
dc.type.rimsART-
dc.citation.volume121-
dc.citation.issue37-
dc.citation.publicationnameProceedings of the National Academy of Sciences of the United States of America-
dc.identifier.doi10.1073/pnas.2407455121-
dc.contributor.localauthorLee, Sang Yup-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorfermentation-
dc.subject.keywordAuthormagnesium transporter-
dc.subject.keywordAuthorMannheimia succiniciproducens-
dc.subject.keywordAuthorsuccinic acid-
dc.subject.keywordAuthorsustainability-
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