DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ji Yeon | ko |
dc.contributor.author | Lee, Jongan | ko |
dc.contributor.author | Ahn, Jung Ho | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2024-09-09T08:00:07Z | - |
dc.date.available | 2024-09-09T08:00:07Z | - |
dc.date.created | 2024-09-09 | - |
dc.date.created | 2024-09-09 | - |
dc.date.issued | 2024-09 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, v.121, no.37 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/322836 | - |
dc.description.abstract | Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Although the importance of magnesium (Mg2+) ion on SA production has been evident from our previous studies, the role of Mg2+ ion remains largely unexplored. In this study, we investigated the impact of Mg2+ ion on SA production and developed a hyper-SA producing strain of M. succiniciproducens by reconstructing the Mg2+ ion transport system. To achieve this, optimal alkaline neutralizer comprising Mg2+ ion was developed and the physiological effect of Mg2+ ion was analyzed. Subsequently, the Mg2+ ion transport system was reconstructed by introducing an efficient Mg2+ ion transporter from Salmonella enterica. A high-inoculum fed-batch fermentation of the final engineered strain produced 152.23 ± 0.99 g/L of SA, with a maximum productivity of 39.64 ± 0.69 g/L/h. These findings highlight the importance of Mg2+ ions and transportation system optimization in succinic acid production by M. succiniciproducens. | - |
dc.language | English | - |
dc.publisher | National Academy of Sciences | - |
dc.title | High-level succinic acid production by overexpressing a magnesium transporter in Mannheimia succiniciproducens | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-85203420205 | - |
dc.type.rims | ART | - |
dc.citation.volume | 121 | - |
dc.citation.issue | 37 | - |
dc.citation.publicationname | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.identifier.doi | 10.1073/pnas.2407455121 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | fermentation | - |
dc.subject.keywordAuthor | magnesium transporter | - |
dc.subject.keywordAuthor | Mannheimia succiniciproducens | - |
dc.subject.keywordAuthor | succinic acid | - |
dc.subject.keywordAuthor | sustainability | - |
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