DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kyungsoo | ko |
dc.contributor.author | Kim, Young Sik | ko |
dc.contributor.author | Jang, Ju Woong | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2024-09-06T07:00:18Z | - |
dc.date.available | 2024-09-06T07:00:18Z | - |
dc.date.created | 2023-12-11 | - |
dc.date.issued | 2024-01 | - |
dc.identifier.citation | BIOTECHNOLOGY JOURNAL, v.19, no.1 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.uri | http://hdl.handle.net/10203/322802 | - |
dc.description.abstract | To enhance the production of recombinant human transforming growth factor-beta1 (rhTGF-beta 1) in Chinese hamster ovary (CHO) cells, rhTGF-beta 1 was first characterized for endocytosis, signaling pathway, and overall maturation process. The mature rhTGF-beta 1 used for clinical application was internalized into CHO cells and inhibited the growth of CHO cells in a dose-dependent manner. However, mature rhTGF-beta 1 was mostly produced in the form of latent rhTGF-beta 1 in cultures of recombinant CHO (rCHO) cells producing rhTGF-beta 1 (CHO-rhTGF-beta 1). The concentration of active mature rhTGF-beta 1 in the culture supernatant of CHO-rhTGF-beta 1 cells was not high enough to compromise yield. In addition, a significant amount of unprocessed precursors was produced by CHO-rhTGF-beta 1 cells. Overexpression of PACEsol, a soluble form of furin, in CHO-rhTGF-beta 1 cells was effective for the proteolytic cleavage of unprocessed precursors. The highest mature rhTGF-beta 1 concentration (6.4 mu g mL(-1)) was obtained with the PACEsol-expressing clone, which was approximately 45% higher than that of the parental clone (P < 0.01). Thus, a comprehensive understanding of the intrinsic properties of rhTGF-beta 1 with respect to the overall maturation process, signaling pathway, and endocytosis is essential for effectively enhancing the production of mature rhTGF-beta 1 in CHO cells. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Enhancing the production of recombinant human TGF-β1 through an understanding of TGF-β1 synthesis, signaling, and endocytosis in CHO cells | - |
dc.type | Article | - |
dc.identifier.wosid | 001109768700001 | - |
dc.identifier.scopusid | 2-s2.0-85178362195 | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | BIOTECHNOLOGY JOURNAL | - |
dc.identifier.doi | 10.1002/biot.202300269 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Kim, Young Sik | - |
dc.contributor.nonIdAuthor | Jang, Ju Woong | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CHO cell | - |
dc.subject.keywordAuthor | recombinant human TGF-beta 1 | - |
dc.subject.keywordAuthor | TGF-beta 1 endocytosis | - |
dc.subject.keywordAuthor | TGF-beta 1 signaling | - |
dc.subject.keywordAuthor | TGF-beta 1 synthesis | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | LATENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PRECURSOR | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FACTOR-BETA-1 | - |
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