DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 신의철 | - |
dc.contributor.author | Jang, Ho Cheol | - |
dc.contributor.author | 장호철 | - |
dc.date.accessioned | 2024-08-08T19:31:28Z | - |
dc.date.available | 2024-08-08T19:31:28Z | - |
dc.date.issued | 2024 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1099365&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/322117 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 의과학대학원, 2024.2,[v, 54 p. :] | - |
dc.description.abstract | IL-7 is a cytokine known to play a key role in the survival, proliferation and differentiation of peripheral blood T cells. This makes it an attractive candidate for therapeutic applications, particularly in conditions characterized by immunocompromised individuals. IL-7 has been used in clinical trials for various conditions associated with lymphopenia, but the exact mechanisms of its effects on peripheral blood T cells have remained unclear. Single-cell transcriptome analysis allows the analysis of individual cells, allowing the identification of transcriptomic heterogeneity and the specific effects of IL-7 at the single cell level in cancer patients. Cancer patients received a single dose of recombinant IL-7 and their peripheral blood T cells were analyzed one week and three weeks after injection. One week after recombinant IL-7 injection, peripheral blood T cells showed decreased expression of genes and transcription factors that maintain cellular quiescence. Most cells showed an increase in cellular activity. In addition, the proportion of proliferating T cells in the total number of T cells increased. However, when recombinant IL-7 was administered 3 weeks later, the effects observed after one week appeared to diminish, suggesting possible desensitization or some other mechanism of adaptation. A decrease in signaling downstream of STAT5 and reduced expression of the transcription factor c-Myc, which is critical for cell proliferation, were observed. The reduced response to IL-7 observed three weeks after injection may be due to changes in the gene expression profile of peripheral blood T cells. Single-cell RNA sequencing shows that the transcriptomic landscape was different before and 3 weeks after IL-7 treatment. This study provides an in-depth analysis of the IL-7 response in human T cells and suggests the mechanism of the decreased response with repeated use of IL-7. Future studies may need to explore ways to improve the efficacy of IL-7 through repeated use. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 인터류킨-7 (IL-7)▼a재조합 IL-7▼aT 세포 항상성▼aIL-7 신호 전달▼aIL-7 저반응성 | - |
dc.subject | Interleukin-7 (IL-7)▼aRecombinant IL-7▼aT cell homeostasis▼aIL-7 signal transduction▼aIL-7 hypo-responsiveness | - |
dc.title | (A) single-cell transcriptome analysis of the effects of exogenous interleukin-7 on peripheral blood T cells in patients with malignancy | - |
dc.title.alternative | 암환자에서 외인성 인터류킨 7이 말초 혈액 T 세포에 미치는 영향에 대한 단일 세포 전사체 분석 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :의과학대학원, | - |
dc.contributor.alternativeauthor | Shin, Eui-Cheol | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.