DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 허원도 | - |
dc.contributor.author | Son, Seungkyu | - |
dc.contributor.author | 손승규 | - |
dc.date.accessioned | 2024-08-08T19:31:12Z | - |
dc.date.available | 2024-08-08T19:31:12Z | - |
dc.date.issued | 2024 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1099257&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/322045 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2024.2,[iii, 66 p. :] | - |
dc.description.abstract | The structural plasticity of synapses is a fundamental mechanism regulating brain functions. However, currently available methods, based on split fluorescent proteins (FPs), have been limited in assessing synaptic dynamics in vivo due to the irreversible binding of split FPs. Here, we develop ‘SynapShot’—a method for visualizing the structural dynamics of intact synapses by combining dimerization-dependent fluorescent proteins (ddFPs) with engineered synaptic adhesion molecules. SynapShot allows real-time monitoring of reversible and bi-directional changes of synaptic contacts under physiological stimulation. The application of green and red ddFPs in SynapShot enables the simultaneous visualization of two distinct populations of synapses. Notably, the red-shifted SynapShot is highly compatible with blue light-based optogenetic techniques, allowing for the visualization of synaptic dynamics while precisely controlling specific signaling pathways. Furthermore, we demonstrate that SynapShot enables real-time monitoring of structural changes in synaptic contacts in the mouse brain during both primitive and higher-order behaviors. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 시냅스▼a이합체화 의존 형광 단백질▼a시냅스 접착 분자▼a광유전학▼a실시간 모니터링▼a마우스의 행동▼a시냅샷 | - |
dc.subject | Synapse▼aDimerization-dependent fluorescent proteins▼aSynaptic adhesion molecule▼aOptogenetics▼aReal-time monitoring▼aMouse behavior▼aSynapShot | - |
dc.title | Real-time visualization of structural dynamics of synapses in live cells in vivo | - |
dc.title.alternative | 살아있는 세포와 동물에서 시냅스의 실시간 시각화를 위한 방법 개발 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | Heo, Won Do | - |
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