DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yu, Jeongmin | ko |
dc.contributor.author | Im, Hyeryeon | ko |
dc.contributor.author | Lee, Gwangrog | ko |
dc.date.accessioned | 2024-08-07T20:00:06Z | - |
dc.date.available | 2024-08-07T20:00:06Z | - |
dc.date.created | 2024-08-08 | - |
dc.date.created | 2024-08-08 | - |
dc.date.created | 2024-08-08 | - |
dc.date.issued | 2023-08 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.668, pp.35 - 41 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/321756 | - |
dc.description.abstract | The recent outbreak of COVID-19 has created a serious health crisis with fatFal infectious viral diseases, such as Severe Acute Respiratory Syndrome (SARS). The nsp13, a helicase of coronaviruses is an essential element for viral replication that unwinds secondary structures of DNA and RNA, and is thus considered a major therapeutic target for treatment. The replication of coronaviruses and other retroviruses occurs in the cytoplasm of infected cells, in association with viral replication organelles, called virus-induced cytosolic double-membrane vesicles (DMVs). In addition, an increase in cytosolic Ca2+ concentration accelerates viral replication. However, the molecular mechanism of nsp13 in the presence of Ca2+ is not well understood. In this study, we applied biochemical methods and single-molecule techniques to demonstrate how nsp13 achieves its unwinding activity while performing ATP hydrolysis in the presence of Ca2+. Our study found that nsp13 could efficiently unwind double stranded (ds) DNA under physio-logical concentration of Ca2+ of cytosolic DMVs. These findings provide new insights into the properties of nsp13 in the range of calcium in cytosolic DMVs. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Unwinding mechanism of SARS-CoV helicase (nsp13) in the presence of Ca2+, elucidated by biochemical and single-molecular studies | - |
dc.type | Article | - |
dc.identifier.wosid | 001009252100001 | - |
dc.identifier.scopusid | 2-s2.0-85159827555 | - |
dc.type.rims | ART | - |
dc.citation.volume | 668 | - |
dc.citation.beginningpage | 35 | - |
dc.citation.endingpage | 41 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2023.05.062 | - |
dc.contributor.localauthor | Lee, Gwangrog | - |
dc.contributor.nonIdAuthor | Yu, Jeongmin | - |
dc.contributor.nonIdAuthor | Im, Hyeryeon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | nsp13 | - |
dc.subject.keywordAuthor | Helicase | - |
dc.subject.keywordAuthor | Unwinding activity | - |
dc.subject.keywordAuthor | Double-membrane vesicles | - |
dc.subject.keywordAuthor | Calcium | - |
dc.subject.keywordAuthor | Single-molecule | - |
dc.subject.keywordPlus | CORONAVIRUS | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | DNA | - |
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