DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박제균 | - |
dc.contributor.author | Han, Jieun | - |
dc.contributor.author | 한지은 | - |
dc.date.accessioned | 2024-07-25T19:30:12Z | - |
dc.date.available | 2024-07-25T19:30:12Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1044781&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/320381 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2022.2,[vi, 55 p. :] | - |
dc.description.abstract | Since amino acid analysis is crucial for monitoring and detecting metabolic states, rapid and easy methods are in high demand. Various metabolic diseases caused by the accumulation of specific amino acids due to abnormal metabolic pathways are fatal and can be diagnosed by measuring the level of amino acids in biological fluids. However, several techniques capable of performing amino acid analysis require complex experimental setup, expensive equipment, and long processing time. Here, we introduce a power-free, portable, and efficient microfluidic device for amino acid quantification using a cell-free system, which is essential for fast processing and direct manipulation. In this platform, the working principle of a finger-actuated pumping unit is applied, and multiple reactions are operated simultaneously with a single button. In addition to the complementary cell-free protein synthesis assay which was previously studied, the device reduces the amount of sample and allows a simple mixing process through a pushbutton. In addition, the reactants that have been reacted in the incubation chambers flow into a detection zone where results can be observed and compared at a glance. Through the platform, the amino acid concentrations can be determined simply by measuring fluorescence signals and also facilitate the diagnosis of amino acid-related diseases. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 다중 반응 미세유체 시스템▼a무동력 미세유체공학▼a무세포 단백질 합성▼a손가락 작동식 장치▼a아미노산 분석▼a현장진단검사 | - |
dc.subject | Amino acid quantification▼aCell-free protein synthesis▼aFinger-actuated device▼aPoint-of-care testing▼aPower-free microfluidics▼aMultiplexed microfluidic system | - |
dc.title | Microfluidic multiplexed device for amino acid quantification using cell-free protein synthesis | - |
dc.title.alternative | 무세포 단백질 합성 반응을 이용한 아미노산 다중 정량용 미세유체소자 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :바이오및뇌공학과, | - |
dc.contributor.alternativeauthor | Park, Je-Kyun | - |
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