DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 조광현 | - |
dc.contributor.author | Joung, Chang Young | - |
dc.contributor.author | 정창영 | - |
dc.date.accessioned | 2024-07-25T19:30:11Z | - |
dc.date.available | 2024-07-25T19:30:11Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1044775&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/320375 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2021.2,[iii, 35 p. :] | - |
dc.description.abstract | Colorectal cancer (CRC) is known to occur through the accumulation of sequential mutations over long periods of time. Because of the high incidence and mortality of CRC, the importance of early diagnosis and treatment has been highlighted. Therefore, it is essential to understand molecular regulatory mechanisms of tumorigenesis in the CRC. In this study, we attempted to identify a master regulator that reverts abnormal gene expression state in CRC to normal gene expression profile. To identify the master regulator of CRC, we reconstructed cancer-specific gene regulatory network (GRN) from CRC patients’ gene expression data. As a result of master regulator analysis (MRA) using GRN, we selected TEAD4 with increased gene expression level and protein activity in adenoma as the master regulator of tumorigenesis. We verified the effect of TEAD4 knockdown (KD) in APC-deleted human colonic epithelial cells (HCEC-1CT-A), a model of CRC development. The results showed that the gene expression profile of HCEC-1CT-A was transformed into normal HCEC-1CT by decreasing proliferation and inducing cell differentiation. However, TEAD4 alone only inhibits cell proliferation in CRC that additional mutations were accumulated. Therefore, in this paper, we suggest ELK1 as a combination target of reverting CRC to normal-like differentiated cells discovered through network analysis. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 대장암▼a유전자 조절 네트워크▼a핵심 조절자▼a전사체 분석 | - |
dc.subject | colorectal cancer▼agene regulatory network▼amaster regulator▼atranscriptome analysis | - |
dc.title | Gene regulatory network analysis identifies master regulators for reverting colorectal cancer cells into normal-like gene expression state | - |
dc.title.alternative | 유전자 조절 네트워크 분석을 통한 대장암의 유전자 발현 정상화 타겟 발굴 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :바이오및뇌공학과, | - |
dc.contributor.alternativeauthor | Cho, Kwang-Hyun | - |
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