Microfluidic Capture Device for Simple, Cell Surface Marker-Based Quantification of Senescent CD8+ T Cells

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 38
  • Download : 0
Among human CD8(+) T cells, senescent cells are marked by the expression of CD57. The frequency of senescent CD57(+)CD8(+) T cells is significantly correlated with aging and age-associated disorders, and it can be measured by multi-color flow cytometry. However, multi-color flow cytometry presents challenges in terms of accessibility and requires significant resource allocation. Therefore, developing a rapid and straightforward method for quantifying CD57(+)CD8(+) T cells remains a key challenge. This study introduces a microfluidic device composed of a PDMS microfluidic channel with a pre-modified glass substrate for anti-CD8 antibody immobilization. This design allows blood samples to flow through, enabling the selective capture of CD8(+) T cells while minimizing the required blood sample volume. This technology enables accurate and reliable quantification of CD57(+) cells among captured CD8(+) T cells through fluorescence image analysis. The ability of the device to easily quantify senescent CD57(+)CD8(+) T cells is anticipated to contribute significantly to both immunological research and clinical applications.
Publisher
Springer
Issue Date
2024-03
Language
English
Article Type
Article; Early Access
Citation

BioChip Journal

ISSN
1976-0280
DOI
10.1007/s13206-024-00142-6
URI
http://hdl.handle.net/10203/319739
Appears in Collection
MSE-Journal Papers(저널논문)PH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0