DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Min-Kyung | ko |
dc.contributor.author | Chang, Jeeyoon | ko |
dc.contributor.author | Park, Joori | ko |
dc.contributor.author | Lee, Hyuk-Joon | ko |
dc.contributor.author | Woo, Jae-Sung | ko |
dc.contributor.author | Kim, Yoon Ki | ko |
dc.date.accessioned | 2024-06-10T03:00:23Z | - |
dc.date.available | 2024-06-10T03:00:23Z | - |
dc.date.created | 2024-06-10 | - |
dc.date.created | 2024-06-10 | - |
dc.date.issued | 2024-04 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.47, no.4 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/319701 | - |
dc.description.abstract | Translation of messenger ribonucleic acids (mRNAs) encoding integral membrane proteins or secreted proteins occurs on the surface of the endoplasmic reticulum (ER). When a nascent signal peptide is synthesized from the mRNAs, the ribosome-nascent chain complex (RNC) is recognized by the signal recognition particle (SRP) and then transported to the surface of the ER. The appropriate targeting of the RNC-SRP complex to the ER is monitored by a quality control pathway, a nuclear cap-binding complex (CBC)-ensured translational repression of RNC-SRP (CENTRE). In this study, using ribosome profiling of CBC-associated and eukaryotic translation initiation factor 4E-associated mRNAs, we reveal that, at the transcriptomic level, CENTRE is in charge of the translational repression of the CBC-RNC-SRP until the complex is specifically transported to the ER. We also find that CENTRE inhibits the nonsense-mediated mRNA decay (NMD) of mRNAs within the CBC-RNC-SRP. The NMD occurs only after the CBC-RNC-SRP is targeted to the ER and after eukaryotic translation initiation factor 4E replaces CBC. Our data indicate dual surveillance for properly targeting mRNAs encoding integral membrane or secretory proteins to the ER. CENTRE blocks gene expression at the translation level before the CBC-RNC-SRP delivery to the ER, and NMD monitors mRNA quality after its delivery to the ER. (c) 2024 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Nonsense-mediated mRNA decay of mRNAs encoding a signal peptide occurs primarily after mRNA targeting to the endoplasmic reticulum | - |
dc.type | Article | - |
dc.identifier.wosid | 001229299200001 | - |
dc.identifier.scopusid | 2-s2.0-85190290276 | - |
dc.type.rims | ART | - |
dc.citation.volume | 47 | - |
dc.citation.issue | 4 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.localauthor | Kim, Yoon Ki | - |
dc.contributor.nonIdAuthor | Shin, Min-Kyung | - |
dc.contributor.nonIdAuthor | Park, Joori | - |
dc.contributor.nonIdAuthor | Lee, Hyuk-Joon | - |
dc.contributor.nonIdAuthor | Woo, Jae-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Cap-binding complex | - |
dc.subject.keywordAuthor | Endoplasmic reticulum | - |
dc.subject.keywordAuthor | Eukaryotic translation initiation factor 4E | - |
dc.subject.keywordAuthor | Nonsense-mediated messenger | - |
dc.subject.keywordAuthor | RNA decay | - |
dc.subject.keywordAuthor | Signal recognition particle | - |
dc.subject.keywordPlus | EXON JUNCTION COMPLEX | - |
dc.subject.keywordPlus | CAP-BINDING COMPLEX | - |
dc.subject.keywordPlus | RECOGNITION PARTICLE | - |
dc.subject.keywordPlus | MECHANISTIC INSIGHTS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | SRP INTERACTION | - |
dc.subject.keywordPlus | QUALITY-CONTROL | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
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