DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Choi, Byong-Seok | - |
dc.contributor.advisor | 최병석 | - |
dc.contributor.author | Kim, Kyoung-Ah | - |
dc.contributor.author | 김경아 | - |
dc.date.accessioned | 2011-12-13T04:48:21Z | - |
dc.date.available | 2011-12-13T04:48:21Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=173590&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/31896 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 화학과, 2002.2, [ viii, 56 p. ] | - |
dc.description.abstract | The ubiqutin (Ub)-like domain of hHR23B is known to specifically interact with S5a, a subunit of 26S proteasome complex. Since hHR23B is one of necessary factor in DNA repair systems, such an interaction might play a role in the regulation of the DNA repair process. The structural studies of the ub-like domain of hHR23B using nuclear magnetic resonance (NMR) spectroscopy was attempted in this study to delineate the specific interaction between the Ub-like domain of hHR23B and S5a. Ub-like domain of hHR23B with a N-terminal six histidine tag overexpressed as a soluble form in E.coli. Binding assay confirmed the interaction between GST-S5a (residues of 263 to 342). Protein backbone resonances of $C^\alpha$, $C^\beta$, CO, $N^H$, and $H^N$ were fully assigned using several hetero-nuclear NMR spectroscopy ; CBCA(CO)NH, HNCACB, $1^H-{15}^N HSQC$, $1^H-{13}^C$ HSQC and HN(CO). Unlike the ubiquitin protein family, featuring the $\beta \beta \alpha \beta \beta \alpha \beta$ fold of ubiquitin, Ub-like domain of hHR23B has $\beta \beta \alpha \beta \alpha \beta \beta$. Furthermore, Ub-like domain in hHR23B has additional eight amino acids to ubiquitin at C-terminus. These characteristics seem to explain the cause that Ub-like domain of hHR23B has specific interaction to S5a. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 26S proteasome (S5a) | - |
dc.subject | NMR | - |
dc.subject | hHR23B | - |
dc.subject | ubiquitin-like domain | - |
dc.subject | structure | - |
dc.subject | 구조 | - |
dc.subject | 26S proteasome (S5a) | - |
dc.subject | 자기공명연구 | - |
dc.subject | hHR23B | - |
dc.subject | ubiquitin 유사 도메인 | - |
dc.title | NMR study on ubiquitin-like domain of hHR23B | - |
dc.title.alternative | hHR23B ubiquitin 유사 도메인의 구조 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 173590/325007 | - |
dc.description.department | 한국과학기술원 : 화학과, | - |
dc.identifier.uid | 020003051 | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.localauthor | 최병석 | - |
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