싸이토카인에 의한 췌장 β세포 독성에 대한 동충하초 추출물의 보호 효과

Abstract

In the present study, Cordyceps sinensis extract (CSE) was evaluated to determine if it could protect pancreatic β cells against cytokine-induced cytotoxicity of RINm5F cells. Treatment of cells with cytokines resulted in a decrease of viability, which was caused by increase of nitric oxide (NO) production. CSE protected cytokine-mediated viability reduction in a concentration-dependent manner. Incubation with CSE also induced a significant suppression of cytokine-induced inducible nitric oxide synthase (iNOS) protein and NO production. The molecular mechanism by which CSE inhibited iNOS protein expression appeared to involve the inhibition of NF-κB activation. The cytokine-stimulated RIN cells showed increases in NF-κB binding activity compared to unstimulated cells. However, pretreatment with CSE inhibited cytokines-induced NF-κB activation in RINm5F cells.