DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhou, Lu | ko |
dc.contributor.author | Yoon, Sun Jung | ko |
dc.contributor.author | Jang, Kyu Yun | ko |
dc.contributor.author | Moon, Young Jae | ko |
dc.contributor.author | Wagle, Sajeev | ko |
dc.contributor.author | Lee, Kwang Bok | ko |
dc.contributor.author | Park, Byung-Hyun | ko |
dc.contributor.author | Kim, Jung Ryul | ko |
dc.date.accessioned | 2024-03-22T07:01:55Z | - |
dc.date.available | 2024-03-22T07:01:55Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | PLOS ONE, v.9, no.10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318792 | - |
dc.description.abstract | Angiogenesis is considered essential for proper bone regeneration. The purpose of this investigation was to determine if a combined therapy of bone morphogenetic protein-2 (BMP-2) and cartilage oligomeric matrix protein angiopoietin-1 (COMP-Ang1) can potentiate the therapeutic effect of BMP-2 in a rat model of ischemic necrosis of the femoral head (INFH). INFH was surgically induced in the femoral head of rats, and the animals were divided into the following groups: 1) a sham-operated group (sham group), 2) a bovine serum albumin-injected group (BSA group), 3) a BMP-2-injected group (BMP-2 group), and 4) a COMP-Ang1 and BMP-2-injected group (COMP-Ang1 + BMP-2 group) (n = 20/group). Radiologic, histologic, and histomorphometric assessments were performed to assess femoral head morphology, vascular density, and bone resorption activity. Western blots and immunohistochemical staining were performed to evaluate production of BMP-related signaling proteins in C3H10T1/2 cells and tissues. Real-time RT-PCR was performed to investigate expression of the target integrin gene, and the effect of integrin on C3H10T1/2 cells was determined using a cell adhesion assay. Radiographs obtained six weeks after injection revealed better preservation of the architecture of the femoral head in the COMP-Ang1 + BMP-2 group compared with the BSA and BMP-2 groups. Histological findings indicated increased trabecular bone and vascularity and decreased osteoclast bone resorption activity in the COMP-Ang1 + BMP-2 group compared with those in the BSA and BMP-2 groups. The combination of COMP-Ang1 and BMP-2 increased phosphorylation of Smad1/3/5, p38, and Akt. Increased integrin alpha 3 and beta 1 mRNA expression in the COMP-Ang1 + BMP-2 group promoted cell adhesion. These results suggest that COMP-Ang1 preserved the necrotic femoral head through the potentiation of BMP-2 signaling pathways and angiogenesis. Combination treatment with COMP-Ang1 and BMP-2 may be a clinically useful therapeutic application in INFH. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | COMP-Angiopoietin1 Potentiates the Effects of Bone Morphogenic Protein-2 on Ischemic Necrosis of the Femoral Head in Rats | - |
dc.type | Article | - |
dc.identifier.wosid | 000345204100093 | - |
dc.identifier.scopusid | 2-s2.0-84908126118 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 10 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0110593 | - |
dc.contributor.localauthor | Park, Byung-Hyun | - |
dc.contributor.nonIdAuthor | Zhou, Lu | - |
dc.contributor.nonIdAuthor | Yoon, Sun Jung | - |
dc.contributor.nonIdAuthor | Jang, Kyu Yun | - |
dc.contributor.nonIdAuthor | Moon, Young Jae | - |
dc.contributor.nonIdAuthor | Wagle, Sajeev | - |
dc.contributor.nonIdAuthor | Lee, Kwang Bok | - |
dc.contributor.nonIdAuthor | Kim, Jung Ryul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | OSTEOBLAST DIFFERENTIATION | - |
dc.subject.keywordPlus | LONG-TERM | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | COMP-ANG1 | - |
dc.subject.keywordPlus | BMP-2 | - |
dc.subject.keywordPlus | ANGIOPOIETIN-1 | - |
dc.subject.keywordPlus | OSTEONECROSIS | - |
dc.subject.keywordPlus | REGENERATION | - |
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