DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, See-Hyoung | ko |
dc.contributor.author | Lee, Jongsung | ko |
dc.contributor.author | Kang, Mi-Ae | ko |
dc.contributor.author | Moon, Young Jae | ko |
dc.contributor.author | Wang, Sung Il | ko |
dc.contributor.author | Kim, Kyoung Min | ko |
dc.contributor.author | Park, Byung-Hyun | ko |
dc.contributor.author | Jang, Kyu Yun | ko |
dc.contributor.author | Kim, Jung Ryul | ko |
dc.date.accessioned | 2024-03-22T06:03:18Z | - |
dc.date.available | 2024-03-22T06:03:18Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.478, no.3, pp.1409 - 1415 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/318752 | - |
dc.description.abstract | Angiogenesis is closely associated with osteoblast differentiation. Previously, we demonstrated that bone formation can be accelerated by treatment with COMP-Angiopoietin1, a known angiogenic factor. Angiopoietin1 (Ang1) is a specific growth factor that generates stable and mature vasculature through the Tie2 receptor. In this study, we aimed to identify a novel drug that can activate endogenous Ang1 expression as a pharmacological treatment for bone formation. Therefore, Ang1 expression was examined in U2OS osteoblast-like cells treated with 770 drugs from a library of Food and Drug Administration (FDA)-approved drugs by using ELISA for Ang1. L-thyroxine was selected as a novel drug candidate. LThyroxine is a synthetic form of the hormone thyroxine, which is used to treat patients with hypothyroidism. Enzyme-linked immunosorbent assays (ELISAs) were performed to test whether Ang1 is induced in a dose-dependent manner in human osteoblast-like cell lines, U2OS and MG63. The effects of L-thyroxine on osteoblast differentiation and mineralization were evaluated by alkaline phosphatase (ALP) activity and Alizarin red s staining. To determine the molecular mechanism, the expression of proteins related to bone formation and differentiation, such as type I collagen (COL1A1), osteocalcin (OC), bone sialoprotein (BSP), distal-less homeobox 5 (Dlx5), Runt-related transcription factor 2 (Runx2), osterix (OSX), and ALP, was tested by Western blotting analysis. Consequently, L-thyroxine induced Ang1 expression in a dose-dependent manner in both U2OS and M63 cells, which was confirmed by ELISA and Western blotting. Also, L-thyroxine activated ALP activity in U2OS and MG63 cells as well as ALP expression. Furthermore, L-thyroxine enhanced the expression of COL1A1, Runx2, OC, BSP, Dlx5, and OSX mRNA and proteins. Taken together, we demonstrated that L-thyroxine increased Ang1 expression and induces bone formation, differentiation, and mineralization in U2OS and MG63 cell lines, which suggests that L-thyroxine could be a potential bone production agent. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Potential of L-thyroxine to differentiate osteoblast-like cells via Angiopoietin1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000397715400062 | - |
dc.identifier.scopusid | 2-s2.0-84992734336 | - |
dc.type.rims | ART | - |
dc.citation.volume | 478 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1409 | - |
dc.citation.endingpage | 1415 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.08.137 | - |
dc.contributor.localauthor | Park, Byung-Hyun | - |
dc.contributor.nonIdAuthor | Park, See-Hyoung | - |
dc.contributor.nonIdAuthor | Lee, Jongsung | - |
dc.contributor.nonIdAuthor | Kang, Mi-Ae | - |
dc.contributor.nonIdAuthor | Moon, Young Jae | - |
dc.contributor.nonIdAuthor | Wang, Sung Il | - |
dc.contributor.nonIdAuthor | Kim, Kyoung Min | - |
dc.contributor.nonIdAuthor | Jang, Kyu Yun | - |
dc.contributor.nonIdAuthor | Kim, Jung Ryul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | L-Thyroxine | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Osteoblast | - |
dc.subject.keywordAuthor | Angiopoietin1 | - |
dc.subject.keywordPlus | OSTEOPROGENITOR CELLS | - |
dc.subject.keywordPlus | ARTERIOLAR GROWTH | - |
dc.subject.keywordPlus | DRUG DEVELOPMENT | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PROGRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.