DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, JH | ko |
dc.contributor.author | Park, BH | ko |
dc.contributor.author | Kim, HK | ko |
dc.contributor.author | Park, TS | ko |
dc.contributor.author | Baek, HS | ko |
dc.date.accessioned | 2024-03-22T03:01:59Z | - |
dc.date.available | 2024-03-22T03:01:59Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2002-06 | - |
dc.identifier.citation | MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.192, no.1-2, pp.197 - 203 | - |
dc.identifier.issn | 0303-7207 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318676 | - |
dc.description.abstract | To elucidate the molecular mechanism in relation to vascular supply and osteoporosis, we investigated the effect of hypoxia on Runx2 expression in MG63 cells. Also investigated was expression of type I collagen and osteocalcin, which are regulated by Runx2, alkaline phosphatase (ALPase) to see if they are affected by hypoxia. Quiescent cultures of MG63 cells were exposed to hypoxia (2% O-2) and normoxia (18% O-2) for 24, 48, 72 and 96 h. In cells exposed to hypoxia, reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that mRNA expression of Runx2, type I collagen, osteocalcin, and ALPase were decreased in a time dependent manner to 96 h. Activity of ALPase was also reduced in the same manner. Western blotting showed a marked decrease in Runx2 protein at 96 h in cells under hypoxia compared to normoxia. These data indicate that Runx2 expression in osteoblasts is reduced by hypoxia, and may be a mechanism of osteoporosis by decreased vascular supply. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Hypoxia decreases Runx2/Cbfa1 expression in human osteoblast-like cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000176720600020 | - |
dc.identifier.scopusid | 2-s2.0-0037188828 | - |
dc.type.rims | ART | - |
dc.citation.volume | 192 | - |
dc.citation.issue | 1-2 | - |
dc.citation.beginningpage | 197 | - |
dc.citation.endingpage | 203 | - |
dc.citation.publicationname | MOLECULAR AND CELLULAR ENDOCRINOLOGY | - |
dc.identifier.doi | 10.1016/S0303-7207(02)00036-9 | - |
dc.contributor.localauthor | Park, BH | - |
dc.contributor.nonIdAuthor | Park, JH | - |
dc.contributor.nonIdAuthor | Kim, HK | - |
dc.contributor.nonIdAuthor | Park, TS | - |
dc.contributor.nonIdAuthor | Baek, HS | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Runx2 (Cbfa1/Pebp2 alpha A/AML3) | - |
dc.subject.keywordAuthor | hypoxia | - |
dc.subject.keywordAuthor | osteoblast | - |
dc.subject.keywordPlus | BONE BLOOD-FLOW | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | CBFA1 | - |
dc.subject.keywordPlus | AGE | - |
dc.subject.keywordPlus | OSTEOPOROSIS | - |
dc.subject.keywordPlus | VESSELS | - |
dc.subject.keywordPlus | DENSITY | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | RUNX2 | - |
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