DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, UH | ko |
dc.contributor.author | HAN, MK | ko |
dc.contributor.author | PARK, BH | ko |
dc.contributor.author | KIM, HR | ko |
dc.contributor.author | AN, NH | ko |
dc.date.accessioned | 2024-03-22T01:01:11Z | - |
dc.date.available | 2024-03-22T01:01:11Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 1993-08 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA, v.1178, no.2, pp.121 - 126 | - |
dc.identifier.issn | 0006-3002 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318637 | - |
dc.description.abstract | The function of the ectoenzyme NAD glycohydrolase (NADase) in ADP-ribose uptake from extracellular NAD was studied in human erythrocytes that express relatively high NADase activity (adult erythrocytes) and erythrocytes expressing very low activity (newborn erythrocytes). The rates of ADP-ribose uptake from NAD in human erythrocytes were correlated with their NADase activities. In contrast, there was no significant difference in the rates of ADP-ribose uptake among these cells when incubated with ADP-ribose. These results indicate that ecto-NADase may have a role as supplier of ADP-ribose for its uptake into the cells and that the cleavage of NAD by NADase is necessary for the ADP-ribose uptake by human erythrocytes. From ADP-ribose uptake studies at 37-degrees-C a K(m) of 0.7 +/- 0.05 muM and a V(max) of 2.04 +/- 0.1 pmol/min per mul cell water was found for the uptake of [H-3]ADP-ribose. The thiol-reactive reagents p-chloromercuribenzene sulfonic acid and N-ethylmaleimide inhibited the uptake ADP-ribose with IC50 values of 50 +/- 4 and 750 +/- 25 mM, respectively. Since efflux of [H-3]ADP-ribose was negligible, the ADP-ribose transport system appears to be unidirectional. The unidirectionality was supported by the evidence that transported ADP-ribose was rapidly degraded to AMP which is impermeable to the membrane. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | FUNCTION OF NAD GLYCOHYDROLASE IN ADP-RIBOSE UPTAKE FROM NAD BY HUMAN ERYTHROCYTES | - |
dc.type | Article | - |
dc.identifier.wosid | A1993LT69000001 | - |
dc.identifier.scopusid | 2-s2.0-0027227668 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1178 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 121 | - |
dc.citation.endingpage | 126 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA | - |
dc.identifier.doi | 10.1016/0167-4889(93)90001-6 | - |
dc.contributor.localauthor | PARK, BH | - |
dc.contributor.nonIdAuthor | KIM, UH | - |
dc.contributor.nonIdAuthor | HAN, MK | - |
dc.contributor.nonIdAuthor | KIM, HR | - |
dc.contributor.nonIdAuthor | AN, NH | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | NAD GLYCOHYDROLASE | - |
dc.subject.keywordAuthor | ADP-RIBOSE UPTAKE | - |
dc.subject.keywordAuthor | ADP-RIBOSE TRANSPORT | - |
dc.subject.keywordAuthor | ECTOENZYME | - |
dc.subject.keywordAuthor | (HUMAN) | - |
dc.subject.keywordAuthor | (ERYTHROCYTE) | - |
dc.subject.keywordPlus | RAT-LIVER | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | MEMBRANE | - |
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