DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Byoung-Hoon | ko |
dc.contributor.author | Jang, Kyung-Won | ko |
dc.contributor.author | Yu, Eun-Sil | ko |
dc.contributor.author | Jeong, Hyejeong | ko |
dc.contributor.author | Jeong, Ki-Hun | ko |
dc.date.accessioned | 2023-11-06T05:02:10Z | - |
dc.date.available | 2023-11-06T05:02:10Z | - |
dc.date.created | 2023-11-06 | - |
dc.date.created | 2023-11-06 | - |
dc.date.created | 2023-11-06 | - |
dc.date.created | 2023-11-06 | - |
dc.date.created | 2023-11-06 | - |
dc.date.issued | 2023-10 | - |
dc.identifier.citation | LAB ON A CHIP, v.23, no.21, pp.4701 - 4707 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/314296 | - |
dc.description.abstract | Plasmonic nucleic acid amplification tests demand high-throughput and multi-target detection of infectious diseases as well as short turnaround time and small size for point-of-care molecular diagnostics. Here, we report a multi-channel plasmonic real-time reverse-transcription polymerase chain reaction (mpRT-qPCR) assay for ultrafast and on-chip multi-target detection. The mpRT-qPCR system features two pairs of plasmonic thermocyclers for rapid nanostructure-driven amplification and microlens array fluorescence microscopes for in situ multi-color fluorescence quantification. Each channel shows a physical dimension of 32 mm, 75 mm, and 25 mm in width, length, and thickness. The ultrathin microscopes simultaneously capture four different fluorescence images from two PCR chambers of a single cartridge at a single shot exposure per PCR cycle of four different excitation light sources. The experimental results demonstrate a single assay result of high-throughput amplification and multi-target quantification for RNA-dependent RNA polymerase, nucleocapsid, and human ribonuclease P genes in SARS-CoV-2 RNA detection. The mpRT-PCR increases the number of tests four times over the single RT-PCR and exhibits a short detection time of 15 min for the four RT-PCR reactions. This point-of-care molecular diagnostic platform can reduce false negative results in clinical applications of virus detection and decentralize healthcare facilities with limited infrastructure. A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Single-shot multi-channel plasmonic real-time polymerase chain reaction for multi-target point-of-care testing | - |
dc.type | Article | - |
dc.identifier.wosid | 001084952000001 | - |
dc.identifier.scopusid | 2-s2.0-85175231218 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 21 | - |
dc.citation.beginningpage | 4701 | - |
dc.citation.endingpage | 4707 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/d3lc00687e | - |
dc.contributor.localauthor | Jeong, Ki-Hun | - |
dc.contributor.nonIdAuthor | Jeong, Hyejeong | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.