Parametric optimization for high speed FLIM implementation

Abstract

FLIM(Fluorescence Lifetime Imaging Microscopy) has been considered an effective technique to investigate chemical properties of the specimens, especially of biological samples. Despite of this advantageous trait, researchers in this field have had difficulties applying FLIM to their systems because acquiring an image using FLIM consumes too much time. To increase the FLIM speed, many methodologies have been developed and applied to the system. One of the recent methodologies is an analogue mean delay based FLIM using a PMT and digitizer for image reconstruction. In this system, however, imaging time is largely dependent upon several parameters such as data transfer rate, sampling rate of an A/D converter, and signal width etc. In this paper, such parametric optimization method is introduced for faster acquisition of the image.