DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, In | ko |
dc.contributor.author | Park, Jang Hyun | ko |
dc.contributor.author | Kim, Yumin | ko |
dc.contributor.author | Kwon, Myoung Seung | ko |
dc.contributor.author | Lee, Heung Kyu | ko |
dc.date.accessioned | 2023-09-14T01:02:03Z | - |
dc.date.available | 2023-09-14T01:02:03Z | - |
dc.date.created | 2023-09-13 | - |
dc.date.created | 2023-09-13 | - |
dc.date.created | 2023-09-13 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.citation | STAR PROTOCOLS, v.4, no.2 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | http://hdl.handle.net/10203/312619 | - |
dc.description.abstract | Obesity affects susceptibility to sexually transmitted diseases like genital her-pes, caused by herpes simplex virus (HSV) 2. The y8 T cells in the vagina play a major role in HSV-2 suppression. Here, we present a protocol for inducing HSV-2 infection intravaginally in high-fat diet-induced obese mice. We describe steps for isolating single cells from vaginal tissue and analyzing cells using sin-gle-cell RNA sequencing and flow cytometry. We then detail confirmation of the y8 T cell phenotype in vitro. For complete details on the use and execution of this protocol, please refer to Park et al.1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Protocol for analyzing yd & nbsp;T cells from the vagina of diet-induced obese mice using single-cell RNA and flow cytometry | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-85161334244 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 2 | - |
dc.citation.publicationname | STAR PROTOCOLS | - |
dc.identifier.doi | 10.1016/j.xpro.2023.102278 | - |
dc.contributor.localauthor | Lee, Heung Kyu | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Antibody | - |
dc.subject.keywordAuthor | Cell culture | - |
dc.subject.keywordAuthor | Cell isolation | - |
dc.subject.keywordAuthor | Cell separation/fractionation | - |
dc.subject.keywordAuthor | Flow Cytometry/Mass Cytometry | - |
dc.subject.keywordAuthor | Immunology | - |
dc.subject.keywordAuthor | Sequence analysis | - |
dc.subject.keywordAuthor | Single Cell | - |
dc.subject.keywordPlus | IMMUNITY | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.