Protocol for analyzing yd & nbsp;T cells from the vagina of diet-induced obese mice using single-cell RNA and flow cytometry

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Obesity affects susceptibility to sexually transmitted diseases like genital her-pes, caused by herpes simplex virus (HSV) 2. The y8 T cells in the vagina play a major role in HSV-2 suppression. Here, we present a protocol for inducing HSV-2 infection intravaginally in high-fat diet-induced obese mice. We describe steps for isolating single cells from vaginal tissue and analyzing cells using sin-gle-cell RNA sequencing and flow cytometry. We then detail confirmation of the y8 T cell phenotype in vitro. For complete details on the use and execution of this protocol, please refer to Park et al.1
Publisher
ELSEVIER
Issue Date
2023-06
Language
English
Article Type
Article
Citation

STAR PROTOCOLS, v.4, no.2

ISSN
2666-1667
DOI
10.1016/j.xpro.2023.102278
URI
http://hdl.handle.net/10203/312619
Appears in Collection
MSE-Journal Papers(저널논문)
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