DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Su-Hyung | - |
dc.contributor.advisor | 박수형 | - |
dc.contributor.advisor | Shin, Eui-Cheol | - |
dc.contributor.advisor | 신의철 | - |
dc.contributor.author | Koh, June-Young | - |
dc.date.accessioned | 2023-06-23T19:33:20Z | - |
dc.date.available | 2023-06-23T19:33:20Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1006547&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/309036 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 의과학대학원, 2022.2,[vi, 73 p. :] | - |
dc.description.abstract | The liver provides a unique niche of lymphocytes enriched with a large proportion of innate-like T cells. However, the heterogeneity and functional characteristics of the hepatic T-cell population remain to be fully elucidated. We obtained liver sinusoidal mononuclear cells from the liver perfusate of healthy donors and recipients with hepatitis B virus (HBV)-associated chronic liver disease (CLD) during liver transplantation. We performed a CITE-seq analysis of liver sinusoidal CD45$^+$ cells in combination with TCR-seq and flow cytometry to examine the phenotypes and functions of liver sinusoidal CD8$^+$ T cells. We identified a distinct CD56$^{hi}$CD161$^-$CD8$^+$ T-cell population characterized by NK-related gene expression and uniquely restricted TCR repertoire, and their frequency among the liver sinusoidal CD8$^+$ T-cell population was significantly increased in patients with HBV-CLD. Although CD56$^{hi}$CD161$^-$CD8$^+$ T cells exhibit weak responsiveness to TCR stimulation, CD56$^{hi}$CD161$^-$CD8$^+$ T cells highly expressed various NK receptors, including CD94, KIRs, and NKG2C, and exerted NKG2C-mediated NK-like effector functions even in the absence of TCR stimulation. In addition, CD56$^{hi}$CD161$^-$CD8$^+$ T cells highly respond to innate cytokines, such as IL-12/18 and IL-15, in the absence of TCR stimulation. In summary, the current study found a distinct CD56$^{hi}$CD161$^-$CD8$^+$ T-cell population characterized by NK-like activation by TCR-independent NKG2C ligation. Further studies are required to elucidate the roles of liver sinusoidal CD56$^{hi}$CD161$^-$CD8$^+$ T cells in immune responses to microbial pathogens or liver immunopathology. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | CD8+ T cell▼aLiver▼aNKG2C▼aNK cell receptor▼aNK-like T cell▼aCITE-seq | - |
dc.subject | CD8 T 세포▼a간▼aNKG2C▼aNK 세포 수용체▼aNK 유사 T 세포▼aCITE-시퀀싱 | - |
dc.title | Distinct NK-like characteristics of liver sinusoidal CD56$^{hi}$CD161$^-$CD8$^+$ T Cells | - |
dc.title.alternative | 사람 간 CD56$^{hi}$CD161$^-$CD8$^+$ T 세포의 NK 세포 유사 특성 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :의과학대학원, | - |
dc.contributor.alternativeauthor | 고준영 | - |
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