DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Pilnam | - |
dc.contributor.advisor | 김필남 | - |
dc.contributor.author | Lee, Kyeonglim | - |
dc.date.accessioned | 2023-06-22T19:32:14Z | - |
dc.date.available | 2023-06-22T19:32:14Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1008260&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/308371 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2022.8,[viii, 38 p. :] | - |
dc.description.abstract | Extracellular matrix (ECM) is a non-cellular tissue architecture containing biophysical and biochemical cues and functions as a reservoir of various biomolecules. Extracellular vesicles (EVs) are important mediators for intercellular communications containing biomolecules such as microRNAs, lipids, and proteins, and they have been considered to exist in a liquid phase. Recently, a new subpopulation of extracellular vesicles, matrix-bound nanovesicles (MBVs), was discovered and found to be bioactive. However, there is no broad proteomics-based analysis of MBVs although properties of microRNAs and lipids have been revealed. In this study, we isolated MBVs from fibroblast-derived ECM and performed LC-MS/MS-based global proteomic characterization to demonstrate how MBVs and soluble EVs differ in protein contents. We discovered that there were distinct proteomic properties of MBVs compared to soluble EVs in specific proteins, EV transport, and EV-ECM interactions. Therefore, this study is expected to provide a proteomics-basis for future studies on MBVs acting as unique microenvironment stimuli in the ECM. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Extracellular matrix▼aextracellular vesicles▼amatrix-bound nanovesicles▼acell-derived matrix▼aLC-MS/MS | - |
dc.subject | 세포외기질▼a세포외소포▼a기질결합 나노소포▼a세포유래 기질▼a액체 크로마토그래피-텐덤질량분석법 | - |
dc.title | Proteomics-based characterization of matrix-bound nanovesicles from fibroblast-derived ECM | - |
dc.title.alternative | 섬유아세포유래 세포외기질의 기질결합 나노소포의 단백질체학기반 특성화 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :바이오및뇌공학과, | - |
dc.contributor.alternativeauthor | 이경림 | - |
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