DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hafner, Antonina | ko |
dc.contributor.author | Park, Minhee | ko |
dc.contributor.author | Berger, Scott E. | ko |
dc.contributor.author | Murphy, Sedona E. | ko |
dc.contributor.author | Nora, Elphège P. | ko |
dc.contributor.author | Boettiger, Alistair N. | ko |
dc.date.accessioned | 2023-06-08T00:00:08Z | - |
dc.date.available | 2023-06-08T00:00:08Z | - |
dc.date.created | 2023-06-07 | - |
dc.date.created | 2023-06-07 | - |
dc.date.created | 2023-06-07 | - |
dc.date.issued | 2023-05 | - |
dc.identifier.citation | MOLECULAR CELL, v.83, no.9, pp.1377 - 1392 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/307132 | - |
dc.description.abstract | Although population-level analyses revealed significant roles for CTCF and cohesin in mammalian genome organization, their contributions at the single-cell level remain incompletely understood. Here, we used a super-resolution microscopy approach to measure the effects of removal of CTCF or cohesin in mouse embryonic stem cells. Single-chromosome traces revealed cohesin-dependent loops, frequently stacked at their loop anchors forming multi-way contacts (hubs), bridging across TAD boundaries. Despite these bridging interactions, chromatin in intervening TADs was not intermixed, remaining separated in distinct loops around the hub. At the multi-TAD scale, steric effects from loop stacking insulated local chromatin from ultra-long range (>4 Mb) contacts. Upon cohesin removal, the chromosomes were more disordered and increased cell-cell variability in gene expression. Our data revise the TAD-centric understanding of CTCF and cohesin and provide a multi-scale, structural picture of how they organize the genome on the single-cell level through distinct contributions to loop stacking. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Loop stacking organizes genome folding from TADs to chromosomes | - |
dc.type | Article | - |
dc.identifier.wosid | 000999227200001 | - |
dc.identifier.scopusid | 2-s2.0-85153619964 | - |
dc.type.rims | ART | - |
dc.citation.volume | 83 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 1377 | - |
dc.citation.endingpage | 1392 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2023.04.008 | - |
dc.contributor.localauthor | Park, Minhee | - |
dc.contributor.nonIdAuthor | Hafner, Antonina | - |
dc.contributor.nonIdAuthor | Berger, Scott E. | - |
dc.contributor.nonIdAuthor | Murphy, Sedona E. | - |
dc.contributor.nonIdAuthor | Nora, Elphège P. | - |
dc.contributor.nonIdAuthor | Boettiger, Alistair N. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CHROMATIN DOMAINS | - |
dc.subject.keywordPlus | COHESIN | - |
dc.subject.keywordPlus | ENHANCER | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | BOUNDARIES | - |
dc.subject.keywordPlus | SEQ | - |
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