Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4(+) T cells

Cited 1 time in webofscience Cited 0 time in scopus
  • Hit : 91
  • Download : 0
CD4(+) T cells play major roles in the adaptive immune system, which requires antigen recognition, costimulation, and cytokines for its elaborate orchestration. Recent studies have provided new insight into the importance of the supramolecular activation cluster (SMAC), which comprises concentric circles and is involved in the amplification of CD4(+) T cell activation. However, the underlying mechanism of SMAC formation remains poorly understood. Here, we performed single-cell RNA sequencing of CD4(+) T cells left unstimulated and stimulated with anti-CD3 and anti-CD28 antibodies to identify novel proteins involved in their regulation. We found that intraflagellar transport 20 (IFT20), previously known as cilia-forming protein, was upregulated in antibody-stimulated CD4(+) T cells compared to unstimulated CD4(+) T cells. We also found that IFT20 interacted with tumor susceptibility gene 101 (TSG101), a protein that endocytoses ubiquitinated T-cell receptors. The interaction between IFT20 and TSG101 promoted SMAC formation, which led to amplification of AKT-mTOR signaling. However, IFT20-deficient CD4(+) T cells showed SMAC malformation, resulting in reduced CD4(+) T cell proliferation, aerobic glycolysis, and cellular respiration. Finally, mice with T-cell-specific IFT20 deficiency exhibited reduced allergen-induced airway inflammation. Thus, our data suggest that the IFT20-TSG101 axis regulates AKT-mTOR signaling via SMAC formation.
Publisher
CHIN SOCIETY IMMUNOLOGY
Issue Date
2023-04
Language
English
Article Type
Article
Citation

CELLULAR & MOLECULAR IMMUNOLOGY, v.20, pp.529 - 539

ISSN
1672-7681
DOI
10.1038/s41423-023-01008-x
URI
http://hdl.handle.net/10203/306996
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 1 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0