DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Changin | ko |
dc.contributor.author | Kim, Yeeun | ko |
dc.contributor.author | Lee, Sang Jin | ko |
dc.contributor.author | Yun, So Ri | ko |
dc.contributor.author | Choi, Jungkweon | ko |
dc.contributor.author | Kim, Seong Ok | ko |
dc.contributor.author | Yang, Yongsoo | ko |
dc.contributor.author | Ihee, Hyotcherl | ko |
dc.date.accessioned | 2023-05-13T07:00:19Z | - |
dc.date.available | 2023-05-13T07:00:19Z | - |
dc.date.created | 2023-05-12 | - |
dc.date.created | 2023-05-12 | - |
dc.date.created | 2023-05-12 | - |
dc.date.issued | 2023-04 | - |
dc.identifier.citation | NANO LETTERS, v.23, no.8, pp.3334 - 3343 | - |
dc.identifier.issn | 1530-6984 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306822 | - |
dc.description.abstract | Obtaining the heterogeneous conformation of small proteins is important for understanding their biological role, but it is enables the observation of heterogeneous conformations of small proteins and applied it to calmodulin. By imaging the proteins labeled by two gold nanoparticles at multiple tilt angles and analyzing the projected positions of the nanoparticles, the distributions of 3D interparticle distances were obtained. From the measured distance distributions, the conformational changes associated with Ca2+ binding and salt concentration were determined. MT-NACS was also used to track the structural change accompanied by the interaction between amyloid-beta and calmodulin, which has never been observed experimentally. This work offers an alternative platform for studying the functional flexibility of small proteins. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Visualizing Heterogeneous Protein Conformations with Multi-Tilt Nanoparticle-Aided Cryo-Electron Microscopy Sampling | - |
dc.type | Article | - |
dc.identifier.wosid | 000974399900001 | - |
dc.identifier.scopusid | 2-s2.0-85154031516 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 3334 | - |
dc.citation.endingpage | 3343 | - |
dc.citation.publicationname | NANO LETTERS | - |
dc.identifier.doi | 10.1021/acs.nanolett.3c00313 | - |
dc.contributor.localauthor | Yang, Yongsoo | - |
dc.contributor.localauthor | Ihee, Hyotcherl | - |
dc.contributor.nonIdAuthor | Choi, Jungkweon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cryo-electron microscopy | - |
dc.subject.keywordAuthor | small -protein heterogeneous conformation | - |
dc.subject.keywordAuthor | gold nanoparticle labeling | - |
dc.subject.keywordAuthor | tilt series analysis | - |
dc.subject.keywordAuthor | calmodulin | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | PARAMECIUM-TETRAURELIA CALMODULIN | - |
dc.subject.keywordPlus | TARGET RECOGNITION | - |
dc.subject.keywordPlus | CALCIUM-IONS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | STATES | - |
dc.subject.keywordPlus | CRYSTAL | - |
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