DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jun, Yong Woong | ko |
dc.contributor.author | Wang, Taejun | ko |
dc.contributor.author | Hwang, Sekyu | ko |
dc.contributor.author | Kim, Dokyoung | ko |
dc.contributor.author | Ma, Donghee | ko |
dc.contributor.author | Kim, Ki Hean | ko |
dc.contributor.author | Kim, Sungjee | ko |
dc.contributor.author | Jung, Junyang | ko |
dc.contributor.author | Ahn, Kyo Han | ko |
dc.date.accessioned | 2023-05-03T07:01:41Z | - |
dc.date.available | 2023-05-03T07:01:41Z | - |
dc.date.created | 2023-05-03 | - |
dc.date.created | 2023-05-03 | - |
dc.date.created | 2023-05-03 | - |
dc.date.created | 2023-05-03 | - |
dc.date.issued | 2018-08 | - |
dc.identifier.citation | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.57, no.32, pp.10142 - 10147 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306523 | - |
dc.description.abstract | Vesicles exchange their contents through membrane fusion processes, kiss-and-run and full-collapse fusion. Indirect observation of these fusion processes using artificial vesicles enhanced our understanding on the molecular mechanisms involved. Direct observation of the fusion processes in a real biological system, however, remains a challenge owing to many technical obstacles. We report a ratiometric two-photon probe offering real-time tracking of lysosomal ATP with quantitative information for the first time. By applying the probe to two-photon live-cell imaging, the lysosomal membrane fusion process in cells has been directly observed and the concentration of its content, lysosomal ATP, has been measured. Results show that the kiss-and-run process between lysosomes proceeds through repeated transient interactions with gradual content mixing, whereas the full-fusion process occurs at once. Furthermore, it is confirmed that both the fusion processes proceed with conservation of the content. Such a small-molecule probe exerts minimal disturbance and hence has potential for studying various biological processes associated with lysosomal ATP. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | A Ratiometric Two-Photon Fluorescent Probe for Tracking Lysosomal ATP: Direct InCellulo Observation of Lysosomal Membrane Fusion Processes | - |
dc.type | Article | - |
dc.identifier.wosid | 000440135700018 | - |
dc.identifier.scopusid | 2-s2.0-85050824312 | - |
dc.type.rims | ART | - |
dc.citation.volume | 57 | - |
dc.citation.issue | 32 | - |
dc.citation.beginningpage | 10142 | - |
dc.citation.endingpage | 10147 | - |
dc.citation.publicationname | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | - |
dc.identifier.doi | 10.1002/anie.201804743 | - |
dc.contributor.localauthor | Jun, Yong Woong | - |
dc.contributor.nonIdAuthor | Wang, Taejun | - |
dc.contributor.nonIdAuthor | Hwang, Sekyu | - |
dc.contributor.nonIdAuthor | Kim, Dokyoung | - |
dc.contributor.nonIdAuthor | Ma, Donghee | - |
dc.contributor.nonIdAuthor | Kim, Ki Hean | - |
dc.contributor.nonIdAuthor | Kim, Sungjee | - |
dc.contributor.nonIdAuthor | Jung, Junyang | - |
dc.contributor.nonIdAuthor | Ahn, Kyo Han | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | fluorescent probes | - |
dc.subject.keywordAuthor | lysosomal ATP | - |
dc.subject.keywordAuthor | membrane fusion | - |
dc.subject.keywordAuthor | ratiometric imaging | - |
dc.subject.keywordAuthor | two-photon microscopy | - |
dc.subject.keywordPlus | KISS-AND-RUN | - |
dc.subject.keywordPlus | NEUROTRANSMITTER RELEASE | - |
dc.subject.keywordPlus | SYNAPTOTAGMIN | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SNARE | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | SECRETION | - |
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