DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Young-Rae | ko |
dc.contributor.author | Byun, Hee Sun | ko |
dc.contributor.author | Won, Minho | ko |
dc.contributor.author | Park, Kyeong Ah | ko |
dc.contributor.author | Kim, Jin Man | ko |
dc.contributor.author | Choi, Byung Lyul | ko |
dc.contributor.author | Lee, Hyunji | ko |
dc.contributor.author | Hong, Jang Hee | ko |
dc.contributor.author | Park, Jongsun | ko |
dc.contributor.author | Seok, Jeong Ho | ko |
dc.contributor.author | Kim, Dong Wook | ko |
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Park, Seung-Kiel | ko |
dc.contributor.author | Hur, Gang Min | ko |
dc.date.accessioned | 2023-04-13T05:00:38Z | - |
dc.date.available | 2023-04-13T05:00:38Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.citation | BMC CANCER, v.8 | - |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306187 | - |
dc.description.abstract | Background: RET/PTC ( rearranged in transformation/papillary thyroid carcinomas) gene rearrangements are the most frequent genetic alterations identified in papillary thyroid carcinoma. Although it has been established that RET/PTC kinase plays a crucial role in intracellular signaling pathways that regulate cellular transformation, growth, and proliferation in thyroid epithelial cells, the upstream signaling that leads to the activation of RET/PTC is largely unknown. Based on the observation of high levels of PLD expression in human papillary thyroid cancer tissues, we investigated whether PLD plays a role in the regulating the RET/ PTC-induced STAT3 activation. Methods: Cancer tissue samples were obtained from papillary thyroid cancer patients ( n = 6). The expression level of PLD was examined using immunohistochemistry and western blotting. Direct interaction between RET/ PTC and PLD was analyzed by co-immunoprecipitation assay. PLD activity was assessed by measuring the formation of [H-3] phosphatidylbutanol, the product of PLD-mediated transphosphatidylation, in the presence of n-butanol. The transcriptional activity of STAT3 was assessed by m67 luciferase reporter assay. Results: In human papillary thyroid cancer, the expression levels of PLD2 protein were higher than those in the corresponding paired normal tissues. PLD and RET/ PTC could be coimmunoprecipitated from cells where each protein was over-expressed. In addition, the activation of PLD by pervanadate triggered phosphorylation of tyrosine 705 residue on STAT-3, and its phosphorylation was dramatically higher in TPC-1 cells ( from papillary carcinoma) that have an endogenous RET/PTC1 than in ARO cells ( from anaplastic carcinoma) without alteration of total STAT-3 expression. Moreover, the RET/PTC- mediated transcriptional activation of STAT-3 was synergistically increased by over-expression of PLD, whereas the PLD activity as a lipid hydrolyzing enzyme was not affected by RET/PTC. Conclusion: These findings led us to suggest that the PLD synergistically functions to activate the STAT3 signaling by interacting directly with the thyroid oncogenic kinase RET/PTC. | - |
dc.language | English | - |
dc.publisher | BMC | - |
dc.title | Modulatory role of phospholipase D in the activation of signal transducer and activator of transcription (STAT)-3 by thyroid oncogenic kinase RET/PTC | - |
dc.type | Article | - |
dc.identifier.wosid | 000256448800001 | - |
dc.identifier.scopusid | 2-s2.0-44949200152 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.publicationname | BMC CANCER | - |
dc.identifier.doi | 10.1186/1471-2407-8-144 | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Kim, Young-Rae | - |
dc.contributor.nonIdAuthor | Byun, Hee Sun | - |
dc.contributor.nonIdAuthor | Won, Minho | - |
dc.contributor.nonIdAuthor | Park, Kyeong Ah | - |
dc.contributor.nonIdAuthor | Kim, Jin Man | - |
dc.contributor.nonIdAuthor | Choi, Byung Lyul | - |
dc.contributor.nonIdAuthor | Lee, Hyunji | - |
dc.contributor.nonIdAuthor | Hong, Jang Hee | - |
dc.contributor.nonIdAuthor | Park, Jongsun | - |
dc.contributor.nonIdAuthor | Seok, Jeong Ho | - |
dc.contributor.nonIdAuthor | Kim, Dong Wook | - |
dc.contributor.nonIdAuthor | Park, Seung-Kiel | - |
dc.contributor.nonIdAuthor | Hur, Gang Min | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | TYROSINE KINASE | - |
dc.subject.keywordPlus | NEOPLASTIC TRANSFORMATION | - |
dc.subject.keywordPlus | PAPILLARY CARCINOMAS | - |
dc.subject.keywordPlus | D ISOZYMES | - |
dc.subject.keywordPlus | CELL-LINE | - |
dc.subject.keywordPlus | V-SRC | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
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