DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Woojung | ko |
dc.contributor.author | Kim, Hyun Jung | ko |
dc.date.accessioned | 2023-03-17T02:00:53Z | - |
dc.date.available | 2023-03-17T02:00:53Z | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS, v.146, pp.135 - 148 | - |
dc.identifier.issn | 0091-679X | - |
dc.identifier.uri | http://hdl.handle.net/10203/305655 | - |
dc.description.abstract | The gut-on-a-chip is a microengineered in vitro model of the living human intestine that reconstitutes the lumen-capillary tissue interface. Intestinal epithelial Caco-2 cells cultured on the extracellular matrix-coated porous membrane in a gut-on-a-chip undergo three-dimensional villus morphogenesis and physiological cytodifferentiation. The gut-on-a-chip closely recapitulates biomechanical and functional characteristics under peristalsis-like cyclic movements and trickling flow, which enables to co-culture gut microbiome with villus epithelium from days to weeks. Host-gut microbiome ecosystem emulated in the gut-on-a-chip allows the pathomimetic modeling of human intestinal diseases such as gut inflammation and bacterial overgrowth. Here, we describe a protocol for microfabrication of a gut-on-a-chip device, reconstitution of intestinal microenvironment, recreation of host-gut microbiome intercellular interactions, and demonstration of the pathophysiology of representative human intestinal diseases associated with the gut microbiome. The modeling of intestinal disease pathophysiology on-chip can potentiate the development of patient-specific disease models that can validate the efficacy and safety of novel therapeutic interventions. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ACADEMIC PRESS INC | - |
dc.title | Pathomimetic modeling of human intestinal diseases and underlying host-gut microbiome interactions in a gut-on-a-chip | - |
dc.type | Article | - |
dc.identifier.wosid | 000452411700008 | - |
dc.identifier.scopusid | 2-s2.0-85048977608 | - |
dc.type.rims | ART | - |
dc.citation.volume | 146 | - |
dc.citation.beginningpage | 135 | - |
dc.citation.endingpage | 148 | - |
dc.citation.publicationname | MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS | - |
dc.identifier.doi | 10.1016/bs.mcb.2018.05.006 | - |
dc.contributor.localauthor | Shin, Woojung | - |
dc.contributor.nonIdAuthor | Kim, Hyun Jung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Review; Book Chapter | - |
dc.subject.keywordPlus | DYSBIOSIS | - |
dc.subject.keywordPlus | BOWEL | - |
dc.subject.keywordPlus | HEALTH | - |
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