DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Min-Ho | ko |
dc.contributor.author | Ju, Jung-Hyun | ko |
dc.contributor.author | Heo, Sun-Yeon | ko |
dc.contributor.author | Cho, Jaehoon | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.contributor.author | Kim, Min-Soo | ko |
dc.contributor.author | Kim, Chul-Ho | ko |
dc.contributor.author | Oh, Baek-Rock | ko |
dc.date.accessioned | 2023-03-06T05:01:08Z | - |
dc.date.available | 2023-03-06T05:01:08Z | - |
dc.date.created | 2023-03-06 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.citation | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS, v.16, no.1 | - |
dc.identifier.issn | 2731-3654 | - |
dc.identifier.uri | http://hdl.handle.net/10203/305462 | - |
dc.description.abstract | BackgroundTo support the sustainability of biodiesel production, by-products, such as crude glycerol, should be converted into high-value chemical products. 1,2-propanediol (1,2-PDO) has been widely used as a building block in the chemical and pharmaceutical industries. Recently, the microbial bioconversion of lactic acid into 1,2-PDO is attracting attention to overcome limitations of previous biosynthetic pathways for production of 1,2-PDO. In this study, we examined the effect of genetic engineering, metabolic engineering, and control of bioprocess factors on the production of 1,2-PDO from lactic acid by K. pneumoniae GEM167 with flux enhancement of the oxidative pathway, using glycerol as carbon source.ResultsWe developed K. pneumoniae GEM167 Delta adhE/pBR-1,2PDO, a novel bacterial strain that has blockage of ethanol biosynthesis and biosynthesized 1,2-PDO from lactic acid when glycerol is carbon source. Increasing the agitation speed from 200 to 400 rpm not only increased 1,2-PDO production by 2.24-fold to 731.0 +/- 24.7 mg/L at 48 h but also increased the amount of a by-product, 2,3-butanediol. We attempted to inhibit 2,3-butanediol biosynthesis using the approaches of pH control and metabolic engineering. Control of pH at 7.0 successfully increased 1,2-PDO production (1016.5 +/- 37.3 mg/L at 48 h), but the metabolic engineering approach was not successful. The plasmid in this strain maintained 100% stability for 72 h.ConclusionsThis study is the first to report the biosynthesis of 1,2-PDO from lactic acid in K. pneumoniae when glycerol was carbon source. The 1,2-PDO production was enhanced by blocking the synthesis of 2,3-butanediol through pH control. Our results indicate that K. pneumoniae GEM167 has potential for the production of additional valuable chemical products from metabolites produced through oxidative pathways. | - |
dc.language | English | - |
dc.publisher | BMC | - |
dc.title | Production of 1,2-propanediol from glycerol in Klebsiella pneumoniae GEM167 with flux enhancement of the oxidative pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000926543300001 | - |
dc.identifier.scopusid | 2-s2.0-85147539531 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS | - |
dc.identifier.doi | 10.1186/s13068-023-02269-4 | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Jo, Min-Ho | - |
dc.contributor.nonIdAuthor | Ju, Jung-Hyun | - |
dc.contributor.nonIdAuthor | Heo, Sun-Yeon | - |
dc.contributor.nonIdAuthor | Cho, Jaehoon | - |
dc.contributor.nonIdAuthor | Kim, Min-Soo | - |
dc.contributor.nonIdAuthor | Kim, Chul-Ho | - |
dc.contributor.nonIdAuthor | Oh, Baek-Rock | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | 1 | - |
dc.subject.keywordAuthor | 2-propanediol | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | Glycerol | - |
dc.subject.keywordAuthor | Lactic acid | - |
dc.subject.keywordAuthor | Oxidative pathway | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | 1,3-PROPANEDIOL PRODUCTION | - |
dc.subject.keywordPlus | 2,3-BUTANEDIOL PRODUCTION | - |
dc.subject.keywordPlus | ANAEROBIC FERMENTATION | - |
dc.subject.keywordPlus | SALMONELLA-TYPHIMURIUM | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | CRUDE GLYCEROL | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | ACID | - |
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