A micro-fluidic chip comprises a well for isolating a specimen, the well being arranged in a one- or two-dimension; a specimen-isolating device disposed above the well; an opening and closing device; an inlet for injection the specimen and an outlet for discharging an excess of the injected specimen; and a reagent-injecting passage for injecting a reagent and a reagent-discharging passage for discharging the reagent. A micro-fluidic chip comprises a well (10) for isolating a specimen, the well being arranged in a one- or two-dimension; a specimen-isolating device (20) disposed above the well and movable upwards and downwards; an opening and closing device (30) disposed above the specimen-isolating device and for moving the specimen-isolating device upwards and downwards; an inlet (40) for injection the specimen and an outlet (50) for discharging an excess of the injected specimen; and a reagent-injecting passage for injecting a reagent and a reagent-discharging passage for discharging the reagent.