DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ghiffary, Mohammad Rifqi | ko |
dc.contributor.author | Prabowo, Cindy Pricilia Surya | ko |
dc.contributor.author | Adidjaja, Joshua Julio | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Kim, Hyun Uk | ko |
dc.date.accessioned | 2022-11-28T06:00:53Z | - |
dc.date.available | 2022-11-28T06:00:53Z | - |
dc.date.created | 2022-11-28 | - |
dc.date.created | 2022-11-28 | - |
dc.date.issued | 2022-11 | - |
dc.identifier.citation | METABOLIC ENGINEERING, v.74, pp.121 - 129 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | http://hdl.handle.net/10203/301110 | - |
dc.description.abstract | 13-Alanine is an important 13-amino acid with a growing demand in a wide range of applications in chemical and food industries. However, current industrial production of 13-alanine relies on chemical synthesis, which usually involves harmful raw materials and harsh production conditions. Thus, there has been increasing demand for more sustainable, yet efficient production process of 13-alanine. In this study, we constructed Corynebacterium glutamicum strains for the highly efficient production of 13-alanine through systems metabolic engineering. First, aspartate 1-decarboxylases (ADCs) from seven different bacteria were screened, and the Bacillus subtilis ADC showing the most efficient 13-alanine biosynthesis was used to construct a 13-alanine-producing base strain. Next, genome-scale metabolic simulations were conducted to optimize multiple metabolic pathways in the base strain, including phosphotransferase system (PTS)-independent glucose uptake system and the biosynthesis of key precursors, including oxaloacetate and L-aspartate. TCA cycle was further engineered for the streamlined supply of key precursors. Finally, a putative 13-alanine exporter was newly identified, and its overexpression further improved the 13-alanine production. Fed-batch fermentation of the final engineered strain BAL10 (pBA2_tr18) produced 166.6 g/L of 13-alanine with the yield and productivity of 0.28 g/g glucose and 1.74 g/L/h, respectively. To our knowledge, this production performance corresponds to the highest titer, yield and productivity reported to date for the microbial fermentation. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Systems metabolic engineering of Corynebacterium glutamicum for the efficient production of 13-alanine | - |
dc.type | Article | - |
dc.identifier.wosid | 000882426500001 | - |
dc.identifier.scopusid | 2-s2.0-85141264019 | - |
dc.type.rims | ART | - |
dc.citation.volume | 74 | - |
dc.citation.beginningpage | 121 | - |
dc.citation.endingpage | 129 | - |
dc.citation.publicationname | METABOLIC ENGINEERING | - |
dc.identifier.doi | 10.1016/j.ymben.2022.10.009 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.localauthor | Kim, Hyun Uk | - |
dc.contributor.nonIdAuthor | Adidjaja, Joshua Julio | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Systems metabolic engineering | - |
dc.subject.keywordAuthor | Genome-scale metabolic simulation | - |
dc.subject.keywordAuthor | 13-Alanine | - |
dc.subject.keywordAuthor | Sustainable production | - |
dc.subject.keywordPlus | ASPARTATE-ALPHA-DECARBOXYLASE | - |
dc.subject.keywordPlus | BETA-ALANINE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MODEL | - |
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