Double-stranded RNA induction as a potential dynamic biomarkerfor DNA-demethylating agents

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Hypomethylating agents (HMAs), such as azacitidine and decitabine, induce cancer cell death by demethylating DNAs to promote the expression of tumor-suppressor genes. HMAs also reactivate the transcription of endogenous double-stranded RNAs (dsRNAs) that trigger the innate immune response and subsequent apoptosis via viral mimicry. However, the expression patterns of endogenous dsRNAs and their relevance in the efficacy of HMAs remain largely uninvestigated. Here, we employ amidine-conjugated spiropyran (Am-SP) to examine the dynamic expression pattern of total dsRNAs regulated by HMAs. By analyzing the bone-marrow aspirates of myelodysplastic syndrome or acute myeloid leukemia patients who received the HMAs, we find a dramatic increase in total dsRNA levels upon treatment only in patients who later benefited from the therapy. We further apply our approach in solid tumor cell lines and show that the degree of dsRNA induction correlates with the effectiveness of decitabine in most cases. Notably, when dsRNA induction is accompanied by increased expression of nc886 RNA, decitabine becomes ineffective. Collectively, our study establishes the potential application of monitoring the total dsRNA levels by a small molecule as an analytical method and a dynamic marker to predict the clinical outcome of the HMA therapy. © 2022 The Author(s)
Publisher
CELL PRESS
Issue Date
2022-09
Language
English
Article Type
Article
Citation

MOLECULAR THERAPY-NUCLEIC ACIDS, v.29, pp.370 - 383

ISSN
2162-2531
DOI
10.1016/j.omtn.2022.07.014
URI
http://hdl.handle.net/10203/301043
Appears in Collection
BS-Journal Papers(저널논문)CBE-Journal Papers(저널논문)
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