Effects of Crowding and Confinement of Enzyme in Mesoporous Silicates on Thermal Stability = 메조포러스 실리카에 효소의 고정화로 Confinement Effect 와 Crowding Effect 에 의한 효소의 열적 안정성 향상

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 372
  • Download : 0
Since the enzyme encapsulation in mesoporous silica could mimic the confined and crowded protein environments in a living cell, the immobilized enzymes in mesoporous silicates were investigated as a possible experimental system for estimating the effects of macromolecular crowding in a confined space on protein thermal stability. Glucose oxidase (GOX) was encapsulated in the both mesoporous silicates at the first, MSU-F and SBA-15, as a model enzyme. The pore diameters of both MSU-F (30 nm) and SBA-15 (8 nm) are large enough to encapsulate GOX. SBA-15 is channel type structure while MSU-F has the structure of cellular form as well. Therefore, GOX molecules (5.2 nm × 6.0 nm × 7.7 nm) can be confined in channel type pore structure of SBA-15 as well as crowded in those inside the mesopore structures. The amount of enzyme loading on the silica supports was controlled by varying initial concentrations of enzyme solution at the adsorption step. During pre-determined time of thermal incubation, the silicate entrapping more GOX molecules retained higher activity than that of less enzyme molecules. As the concentrations of free GOX solutions were increased, thermal stability of enzyme inside the pore was also enhanced presumably in virtue of the crowding effect as well as confinement effect. This result suggests that more confined and crowded environment can enhance the thermal stability of enzymes.
Advisors
Park, Hyun-gyuresearcher박현규researcherKim, jung-baeresearcher김중배researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2008
Identifier
301935/325007  / 020064069
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2008. 8., [ 40 p. ]

Keywords

Gluocose Oxidase; Mesoporous silica; Crowding effect; Confinement effect; Thermal stability; 글루코스 옥시데이즈 효소; 메조포러스 실리카; 고농도 효과; 집적효과; 열적 안정성; Gluocose Oxidase; Mesoporous silica; Crowding effect; Confinement effect; Thermal stability; 글루코스 옥시데이즈 효소; 메조포러스 실리카; 고농도 효과; 집적효과; 열적 안정성

URI
http://hdl.handle.net/10203/29974
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=301935&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0