Removal of BSA using solid-phase micro-extraction

Abstract

Serum albumin is one of the most abundant serum proteins which block the expression other important biomarkers. The objective of this study is to remove serum albumin effectively by using solid phase extraction in microfluidic devices. Photo-polymerized adsorbent was used to remove BSA. The adsorption capacity was examined with the effect of pH and concentration in BSA solution, and adjusting the concentration of monomer such as hydrophilic AMPS and AAm in adsorbent. Optimal conditions were applied to solid phase extraction in microchannel. Selective removal in bicomponent with bovine serum albumin and γ-globulin was performed with adjusting pH.