Although membrane contactors (MCs) have been recognized to be an efficient approach for the removal of ammonia from water streams, factors affecting the MCs performance were not clearly investigated. In this study, the effects of stripping solution chemistry (acid types and concentration), feed solution chemistry (pH, temperature, and ammonia concentration), and stages of MCs system have been comprehensively evaluated. Interestingly, the type of stripping solutions significantly affected the removal of ammonia, and the comparative effectiveness were in the order of H3PO4 > H2SO4 > HCOOH. However, the concentration of stripping solutions and ammonia in the feed has little impact to the performance of MCs. Among the feed solution chemistry, pH and temperature were the most crucial factors for ammonia removal in MCs, because the increase of pH and temperature enhanced the free ammonia fraction in the solution and facilitated the mass transfer through pores. At the absorbent concentration of 0.5 M H3PO4, pH of 10, and temperature of 40 °C, single-stage MCs could achieve 51% of ammonia removal within 40 s, and the ammonia removal rate in two-stage MCs reached 90% at the 1.5 min of hydraulic retention time (HRT). The results suggested the superior feasibility of multi-stage MCs system compare to the conventional stripping processes for the removal of ammonia in various waste or wastewater.