Improved production of heme using metabolically engineered Escherichia coli

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Heme has recently attracted much attention due to its promising applications in the food and healthcare industries. However, the current titers and productivities of heme produced by recombinant microorganisms are not high enough for a wide range of applications. In this study, the process for the fermentation of the metabolically engineered Escherichia coli HAEM7 strain was optimized for the high-level production of heme. To improve the production of heme, different carbon sources, iron concentration in the medium, pH control strategies, induction points, and iron content in the feeding solution were examined. Moreover, strategies of increasing cell density, regular iron supplementation, and supply of excess feeding solution were developed to further improve the production of heme. In the optimized fermentation process, the HAEM7 strain produced 1.03 g/L heme with productivity of 21.5 mg/L/h. The fermentation process and strategies reported here will expedite establishing industry-level production of heme.
Publisher
WILEY
Issue Date
2022-11
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.119, no.11, pp.3178 - 3193

ISSN
0006-3592
DOI
10.1002/bit.28194
URI
http://hdl.handle.net/10203/298923
Appears in Collection
RIMS Journal PapersCBE-Journal Papers(저널논문)
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